不同营养物饥饿条件下小球藻产油和虾青素的差异性响应。
Differential responses of the green microalga Chlorella zofingiensis to the starvation of various nutrients for oil and astaxanthin production.
机构信息
Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
出版信息
Bioresour Technol. 2018 Feb;249:791-798. doi: 10.1016/j.biortech.2017.10.090. Epub 2017 Oct 27.
Chlorella zofingiensis has been proposed as a potential producer of lipids and the high-value carotenoid astaxanthin. In this study, the responses of photoautotrophic C. zofingiensis with respect to growth, lipid profiles and astaxanthin accumulation were investigated upon the starvation of N (NS), P (PS) and S (SS). NS and SS stimulated triacylglycerol (TAG) accumulation, which reached 27% and 21% of dry weight (DW), respectively. Stresses also stimulated astaxanthin accumulation greatly, reaching 3.9 mg/g DW by NS. SS led to the highest TAG productivity (52.4 mg L d) while NS gave rise to the highest astaxanthin productivity (0.624 mg L d). In combination with transcriptional analysis, a working model for stress-associated TAG and astaxanthin biosynthesis was proposed. Taken together, these detailed data shed light on the elucidation of differential responses to nutrient stresses and may provide insights into future engineering of this promising alga for improving TAG and astaxanthin production.
已有人提出拟球藻可作为生产脂质和高附加值类胡萝卜素虾青素的潜在生物。本研究探讨了在氮饥饿（NS）、磷饥饿（PS）和硫饥饿（SS）条件下，光自养拟球藻的生长、脂类谱和虾青素积累的响应。NS 和 SS 刺激三酰基甘油（TAG）积累，分别达到干重（DW）的 27%和 21%。胁迫还大大刺激了虾青素的积累，NS 条件下虾青素积累量达到 3.9 mg/g DW。SS 导致最高的 TAG 生产力（52.4 mg/L/d），而 NS 则产生最高的虾青素生产力（0.624 mg/L/d）。结合转录分析，提出了与应激相关的 TAG 和虾青素生物合成的工作模型。综上所述，这些详细的数据阐明了对营养胁迫的不同响应，可能为未来对这种有前途的藻类进行工程改造以提高 TAG 和虾青素的生产提供了新的思路。
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