Chen Qiaohong, Chen Yi, Xu Quan, Jin Hu, Hu Qiang, Han Danxiang
Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Front Bioeng Biotechnol. 2022 Feb 24;10:834230. doi: 10.3389/fbioe.2022.834230. eCollection 2022.
has obtained particular interest as a promising candidate for natural astaxanthin production. In this study, we established a two-stage heterotrophic cultivation process, by using which both the growth of . and astaxanthin accumulation are substantially enhanced. Specifically, the ultrahigh biomass concentration of 221.3 g L was achieved under the optimum culture conditions in 7.5 L fermenter during 12 days. When scaled-up in the 500 L fermentor, the biomass yield reached 182.3 g L in 9 days, while the astaxanthin content was 0.068% of DW. To further promote astaxanthin accumulation, gibberellic Acid-3 (GA3) was screened from a variety of phytohormones and was combined with increased C/N ratio and NaCl concentration for induction. When was grown with the two-stage cultivation strategy, the astaxanthin yield reached 0.318 g L, of which the biomass yield was 235.4 g L and astaxanthin content was 0.144% of DW. The content of the total fatty acids increased from 23 to 42% of DW simultaneously. Such an astaxanthin yield was 5.4-fold higher than the reported highest record and surpassed the level of . This study demonstrated that heterotrophic cultivation of is competitive for industrial astaxanthin production.
作为天然虾青素生产的一个有前途的候选者，已引起了特别的关注。在本研究中，我们建立了一个两阶段异养培养过程，通过该过程，[具体生物名称]的生长和虾青素积累都得到了显著提高。具体而言，在7.5升发酵罐中，在最佳培养条件下，12天内实现了221.3克/升的超高生物量浓度。当在500升发酵罐中放大培养时，9天内生物量产量达到182.3克/升，而虾青素含量为干重的0.068%。为了进一步促进虾青素积累，从多种植物激素中筛选出赤霉素-3（GA3），并将其与提高的碳氮比和氯化钠浓度相结合进行诱导。当[具体生物名称]采用两阶段培养策略生长时，虾青素产量达到0.318克/升，其中生物量产量为235.4克/升，虾青素含量为干重的0.144%。总脂肪酸含量同时从干重的23%增加到42%。这样的虾青素产量比报道的最高记录高5.4倍，超过了[对比对象]的水平。本研究表明，[具体生物名称]的异养培养在工业虾青素生产方面具有竞争力。
