Keiler Annekathrin M, Helle Janina, Bader Manuela I, Ehrhardt Tino, Nestler Kristin, Kretzschmar Georg, Bernhardt Ricardo, Vollmer Günter, Nikolić Dejan, Bolton Judy L, Pauli Guido F, Chen Shao-Nong, Dietz Birgit M, van Breemen Richard B, Zierau Oliver
Institute of Doping Analysis and Sports Biochemistry Dresden, 01731 Kreischa, Germany; Institute of Zoology, Molecular Cell Physiology and Endocrinology, Technische Universität Dresden, 01062 Dresden, Germany.
Institute of Zoology, Molecular Cell Physiology and Endocrinology, Technische Universität Dresden, 01062 Dresden, Germany.
Phytomedicine. 2017 Oct 15;34:50-58. doi: 10.1016/j.phymed.2017.08.001. Epub 2017 Aug 2.
Hops (Humulus lupulus (L.)) dietary supplements are of interest as herbal remedies to alleviate menopausal symptoms, such as hot flushes, depression and anxiety. So far, the evidence regarding estrogenic and related properties of hops preparations has been considered insufficient for a market authorization for menopausal indications.
The study aims to investigate a chemically standardized hops extract regarding its safety in the uterus, as wells as its efficacy to prevent bone loss in the ovariectomized rat model.
STUDY DESIGN/METHODS: Female Wistar rats were ovariectomized and divided into a control group receiving phytoestrogen-free diet, a group treated with Ebenzoate (0.93 mg/kg body weight/d) and a group treated with the standardized hops extract (60 mg/kg body weight/d) for 8 weeks. Micro-computed tomography of the tibiae and vertebrae, as wells as histological changes in the uterus and tibia were analyzed.
Neither uterotrophic nor proliferative effects were observed in the endometrium in response to the oral 8-week administration of the hops extract. However, site-dependent skeletal effects were observed. The hops extract significantly decreased the number of osteoclasts in the tibial metaphysis and prevented reduction of the trabecular thickness that resulted from estradiol depletion. In contrast, the hops extract did not prevent the ovariectomy-induced micro-architectural changes in the lumbar vertebra. Certain parameters (e.g. thickness and number of trabeculae) were even found to be below the values determined in the ovariectomized control group.
Taken together, the results provide evidence for the safety of the standardized hops extract and point to a weak bone type-specific, protective effect on bone loss following estradiol depletion.
啤酒花（Humulus lupulus (L.)）膳食补充剂作为缓解潮热、抑郁和焦虑等更年期症状的草药疗法备受关注。到目前为止，关于啤酒花制剂的雌激素及相关特性的证据被认为不足以支持其用于更年期适应症的市场授权。
本研究旨在调查一种化学标准化的啤酒花提取物在子宫中的安全性，以及其在去卵巢大鼠模型中预防骨质流失的功效。
研究设计/方法：将雌性Wistar大鼠去卵巢，分为接受无植物雌激素饮食的对照组、用苯甲酸雌二醇（0.93毫克/千克体重/天）治疗的组和用标准化啤酒花提取物（60毫克/千克体重/天）治疗的组，持续8周。分析胫骨和椎骨的微计算机断层扫描结果，以及子宫和胫骨的组织学变化。
口服8周啤酒花提取物后，子宫内膜未观察到子宫营养或增殖效应。然而，观察到了部位依赖性的骨骼效应。啤酒花提取物显著减少了胫骨近端干骺端的破骨细胞数量，并防止了因雌二醇耗竭导致的小梁厚度减少。相比之下，啤酒花提取物未能预防去卵巢引起的腰椎微结构变化。甚至发现某些参数（如小梁厚度和数量）低于去卵巢对照组测定的值。
综上所述，结果为标准化啤酒花提取物的安全性提供了证据，并表明其对雌二醇耗竭后的骨质流失具有微弱的骨类型特异性保护作用。
