Teng Hui, Lin Qiyang, Li Kang, Yuan Benyao, Song Hongbo, Peng Hongquan, Yi Lunzhao, Wei Ming-Chi, Yang Yu-Chiao, Battino Maurizio, Cespedes Acuña Carlos L, Chen Lei, Xiao Jianbo
College of Food Science, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, 350002 Fuzhou, China.
Kiang Wu Hospital, Macau, China.
Food Chem Toxicol. 2017 Dec;110:418-424. doi: 10.1016/j.fct.2017.09.010. Epub 2017 Sep 9.
Raspberry seed is a massive byproduct of raspberry juice and wine but usually discarded. The present study employed a microwave-assisted method for extraction of raspberry seed oil (RSO). The results revealed that omega-6 fatty acids (linoleic acid and γ-linolenic acid) were the major constituents in RSO. Cellular antioxidant enzyme activity such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were investigated in HepG2 cells treated with RSO. Induction of the synthesis of several antioxidants in HO-exposed HepG2 cells was found. RSO increased the enzyme activity of SOD, CAT, and GPx in HO-exposed HepG2. Furthermore, RSO inhibited the phosphorylation of upstream mitogen-activated protein kinases (MAPK) such as c-Jun N-terminal kinase (c-JNK) and extracellular signal-regulated kinase (ERK). Taken together, the possible mechanisms to increase antioxidant enzyme activities in HepG2 may through the suppression of ERK and JNK phosphorylation. Raspberry seed oil exhibited good effects on the activities of the intracellular antioxidant enzymes and seems to protect the liver from oxidative stress through the inhibition of MAPKs.
树莓籽是树莓汁和树莓酒的大量副产品，但通常被丢弃。本研究采用微波辅助法提取树莓籽油（RSO）。结果表明，ω-6脂肪酸（亚油酸和γ-亚麻酸）是RSO的主要成分。在用RSO处理的HepG2细胞中研究了细胞抗氧化酶活性，如超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）。发现在HO暴露的HepG2细胞中几种抗氧化剂的合成被诱导。RSO增加了HO暴露的HepG2中SOD、CAT和GPx的酶活性。此外，RSO抑制了上游丝裂原活化蛋白激酶（MAPK）如c-Jun氨基末端激酶（c-JNK）和细胞外信号调节激酶（ERK）的磷酸化。综上所述，增加HepG2中抗氧化酶活性的可能机制可能是通过抑制ERK和JNK磷酸化。树莓籽油对细胞内抗氧化酶的活性表现出良好的作用，并且似乎通过抑制MAPKs来保护肝脏免受氧化应激。
