Department of Pharmacology and Toxicology, Faculty of Medicine, UWM, 10-082 Olsztyn, Poland.
Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland.
Nutrients. 2020 Jun 1;12(6):1630. doi: 10.3390/nu12061630.
This study aimed to evaluate the protective role of ground raspberry seeds (RBS) as a source of polyphenols and essential fatty acids on blood plasma enzymatic antioxidant status, lipid profile, and endothelium-intact vasodilation during physiological and pathological conditions. Young normotensive Wistar-Kyoto rats (WKYs) and spontaneously hypertensive rats (SHRs) at ten weeks of age were fed with either a control diet or were supplemented with added 7% RBS for six weeks ( = 6). The main component of RBS was dietary fiber (64%) and the main polyphenols were ellagitannins (1.2%) and flavan-3-ols (0.45%). Irrespective of the rat model, ground RBS decreased liver enzyme aspartate aminotransferase (0.9-fold) and hydrogen peroxide scavenging capacity (Catalase, 0.9-fold). In supplemented SHRs, preincubation with inducible nitric oxide synthase (iNOS) inhibitor 1400W, nonselective cyclooxygenase (COX) inhibitor indomethacin, selective COX-2 inhibitor NS-398, prostacyclin (PGI) synthesis inhibitor tranylcypromine (TCP), thromboxane receptor (TP) antagonist SQ-29548, thromboxane synthesis inhibitor furegrelate, and 20-HETE synthesis inhibitor HET0016 induced the same relaxant response to acetylcholine as in the nonsupplemented control group. In supplemented WKYs, atherogenic index was decreased (0.8-fold), while iNOS and COX-2-derived PGI increased acetylcholine-induced vasodilation. These effects of ground RBS may constitute a potential mechanism for preventing cardiovascular diseases.
本研究旨在评估覆盆子地面种子（RBS）作为多酚和必需脂肪酸的来源，对生理和病理条件下的血浆酶抗氧化状态、脂质谱和完整内皮血管舒张的保护作用。十周龄的年轻正常血压 Wistar-Kyoto 大鼠（WKYs）和自发性高血压大鼠（SHRs）分别用对照饮食或添加 7%RBS 的饮食喂养六周（n=6）。RBS 的主要成分是膳食纤维（64%），主要的多酚是鞣花单宁（1.2%）和黄烷-3-醇（0.45%）。无论大鼠模型如何，地面 RBS 均降低了肝酶天冬氨酸氨基转移酶（降低 0.9 倍）和过氧化氢清除能力（过氧化氢酶，降低 0.9 倍）。在补充 SHRs 中，预先孵育诱导型一氧化氮合酶（iNOS）抑制剂 1400W、非选择性环氧化酶（COX）抑制剂吲哚美辛、选择性 COX-2 抑制剂 NS-398、前列环素（PGI）合成抑制剂曲尼司特（TCP）、血栓素受体（TP）拮抗剂 SQ-29548、血栓素合成抑制剂 furegrelate 和 20-HETE 合成抑制剂 HET0016，均可引起与非补充对照大鼠组相同的乙酰胆碱舒张反应。在补充 WKYs 中，致动脉粥样硬化指数降低（降低 0.8 倍），而 iNOS 和 COX-2 衍生的 PGI 增加了乙酰胆碱诱导的血管舒张。地面 RBS 的这些作用可能构成预防心血管疾病的潜在机制。
