Vuillez P, Pérez S C, Stoeckel M E
Laboratoire de Physiologie, Université Louis Pasteur, UA 309 C.N.R.S., Strasbourg, France.
Neurosci Lett. 1987 Aug 18;79(1-2):53-8. doi: 10.1016/0304-3940(87)90671-9.
Distribution of the gamma-aminobutyric acid (GABA)ergic and dopaminergic innervations was studied in the rat neurointermediate lobe using antibodies against GABA and tyrosine hydroxylase. In light microscopy, immunoperoxidase staining revealed similar distribution patterns of the axons reacting with both antibodies. Diffusely scattered in both lobes, they were more concentrated along the marginal zone of the neural lobe. Application of a double, recto-verso, immunogold labelling method in electron microscopy revealed systematic colocalization of GABA and tyrosine hydroxylase (TH) immunoreactivities in the axons innervating the intermediate lobe; in the neural lobe, almost all GABA-immunoreactive axons were also labelled for TH. Thus, GABA and dopamine, hitherto reported to occur in distinct axons, in fact colocalize in the axonal systems which innervate the pituitary neurointermediate lobe. These observations suggest possible interactions (pre- or postsynaptic) of both transmitters in the functional regulation of the intermediate and neural lobes.
利用抗γ-氨基丁酸（GABA）和酪氨酸羟化酶的抗体，研究了大鼠神经中间叶中GABA能和多巴胺能神经支配的分布情况。在光学显微镜下，免疫过氧化物酶染色显示，与这两种抗体发生反应的轴突具有相似的分布模式。它们在两个叶中呈弥漫性散在分布，在神经叶的边缘区更为集中。在电子显微镜下应用双标、正反免疫金标记法显示，支配中间叶的轴突中GABA和酪氨酸羟化酶（TH）免疫反应性存在系统性共定位；在神经叶中，几乎所有GABA免疫反应性轴突也被标记为TH。因此，迄今报道分别存在于不同轴突中的GABA和多巴胺，实际上在支配垂体神经中间叶的轴突系统中共定位。这些观察结果提示，这两种递质在中间叶和神经叶的功能调节中可能存在（突触前或突触后）相互作用。
