Médina M, Repérant J, Dufour S, Ward R, Le Belle N, Miceli D
URA 1137, Laboratoire d'Anatomie comparée, Muséum national d'Histoire naturelle, Paris, France.
Anat Embryol (Berl). 1994 Jan;189(1):25-39. doi: 10.1007/BF00193127.
The distribution of GABA-immunoreactivity was studied in the brain of the silver eel (Anguilla anguilla) by means of antibodies directed against GABA. Immunoreactive neuronal somata were distributed throughout the brain. Positive perikarya were detected in the internal cellular layer of the olfactory bulb, and in all divisions of the telencephalon, the highest density being observed along the midline. Numerous GABA-reactive cell bodies were found in the diencephalon, particularly in the preoptic and tuberal regions of the hypothalamus, and the dorsolateral, dorsomedial and ventromedial thalamic nuclei. In the optic tectum, the majority of GABA-positive cell bodies were located in the periventricular layer. A number of immunolabeled cell bodies were observed in different tegmental structures, notably the torus semicircularis. In the cerebellum, the Purkinje cells were either very intensely or very weakly immunoreactive. In the rhombencephalon, reactive cell bodies were observed in the eminentia granularis, the valvula cerebellaris, the octavolateral nucleus, the lobus vagus and in the vagal and glossopharyngeal motor nuclei. Intensely immunoreactive axons and terminals were observed in the external granular layer and internal cellular layer of the olfactory bulb. In the telencephalon, the highest density of reactive fibres and boutons was found in the fields of the medial wall. Many immunolabeled fibres were seen in the medial and lateral forebrain bundles. In the diencephalon, intense labelling of fibres and terminals were observed in the nuclei situated close to the midline. In the optic tectum the highest density of reactive fibres was seen in the sfgs, the layer to which the retina projects massively. Finally, in the rhombencephalon the strongest labelling of neurites was observed in the nuclei of the raphé, the nucleus octavocellularis magnocellularis and the nuclei of the IXth and Xth cranial nerves. The GABAergic system of the eel, which is well developed, appears to be generally comparable to that described in tetrapod vertebrates.
利用抗γ-氨基丁酸（GABA）抗体研究了欧洲鳗鲡（Anguilla anguilla）脑内GABA免疫反应性的分布。免疫反应性神经元胞体分布于整个脑内。在嗅球的内细胞层以及端脑的所有分区均检测到阳性胞体，沿中线处密度最高。间脑内发现大量GABA反应性细胞体，尤其是下丘脑的视前区和结节区，以及背外侧、背内侧和腹内侧丘脑核。在视顶盖，大多数GABA阳性细胞体位于室周层。在不同的被盖结构中观察到一些免疫标记细胞体，特别是半规管隆起。在小脑，浦肯野细胞的免疫反应性要么非常强，要么非常弱。在菱脑，在颗粒隆起、小脑瓣、八分体外侧核、迷走叶以及迷走神经和舌咽神经运动核中观察到反应性细胞体。在嗅球的外颗粒层和内细胞层观察到强烈免疫反应性的轴突和终末。在端脑，反应性纤维和终末密度最高的区域在内侧壁区域。在中脑和外侧前脑束中可见许多免疫标记纤维。在间脑，靠近中线的核团中观察到纤维和终末的强烈标记。在视顶盖，反应性纤维密度最高的区域在视网膜大量投射的视纤维层（sfgs）。最后，在菱脑，在中缝核、大细胞八细胞核以及第九和第十对脑神经核中观察到神经突的最强标记。鳗鲡发育良好的GABA能系统总体上似乎与四足脊椎动物中描述的系统相当。
