Department of Chemistry, Washington University in St. Louis, One Brookings Drive, Saint Louis, MO, 63130, USA.
Angew Chem Int Ed Engl. 2017 Nov 6;56(45):14007-14010. doi: 10.1002/anie.201706697. Epub 2017 Oct 5.
Described is a novel, laser-initiated radical trifluoromethylation for protein footprinting and its broad residue coverage. CF reacts with 18 of the 20 common amino acids, including Gly, Ala, Ser, Thr, Asp, and Glu, which are relatively silent with regard to OH. This new approach to footprinting is a bridge between trifluoromethylation in materials and medicinal chemistry and structural biology and biotechnology. Its application to a membrane protein and to myoglobin show that the approach is sensitive to protein conformational change and solvent accessibility.
描述了一种新型的、激光引发的自由基三氟甲基化方法，用于蛋白质足迹分析及其广泛的残基覆盖。CF 与 20 种常见氨基酸中的 18 种反应，包括 Gly、Ala、Ser、Thr、Asp 和 Glu，它们对 OH 相对沉默。这种将三氟甲基化引入材料化学、药物化学、结构生物学和生物技术的新方法是一种桥梁。将其应用于膜蛋白和肌红蛋白表明，该方法对蛋白质构象变化和溶剂可及性敏感。
