基于蛋白质快速光化学氧化（FPOP）平台的卡宾蛋白质足迹分析
Protein Footprinting by Carbenes on a Fast Photochemical Oxidation of Proteins (FPOP) Platform.
作者信息
Zhang Bojie, Rempel Don L, Gross Michael L
机构信息
Department of Chemistry, Washington University in St. Louis, St. Louis, MO, 63130, USA.
出版信息
J Am Soc Mass Spectrom. 2016 Mar;27(3):552-5. doi: 10.1007/s13361-015-1313-9. Epub 2015 Dec 17.
Abstract
Protein footprinting combined with mass spectrometry provides a method to study protein structures and interactions. To improve further current protein footprinting methods, we adapted the fast photochemical oxidation of proteins (FPOP) platform to utilize carbenes as the footprinting reagent. A Nd-YAG laser provides 355 nm laser for carbene generation in situ from photoleucine as the carbene precursor in a flow system with calmodulin as the test protein. Reversed-phase liquid chromatography coupled with mass spectrometry is appropriate to analyze the modifications produced in this footprinting. By comparing the modification extent of apo and holo calmodulin on the peptide level, we can resolve different structural domains of the protein. Carbene footprinting in a flow system is promising.
摘要
蛋白质足迹分析与质谱联用提供了一种研究蛋白质结构和相互作用的方法。为了进一步改进当前的蛋白质足迹分析方法，我们对蛋白质快速光化学氧化（FPOP）平台进行了改进，以利用卡宾作为足迹分析试剂。钕钇铝石榴石激光在流动系统中提供355 nm激光，用于以光亮氨酸作为卡宾前体原位生成卡宾，以钙调蛋白作为测试蛋白。反相液相色谱与质谱联用适合分析这种足迹分析中产生的修饰。通过在肽水平上比较脱辅基钙调蛋白和全血钙调蛋白的修饰程度，我们可以解析该蛋白质的不同结构域。流动系统中的卡宾足迹分析前景广阔。
相似文献
1
Protein Footprinting by Carbenes on a Fast Photochemical Oxidation of Proteins (FPOP) Platform.基于蛋白质快速光化学氧化（FPOP）平台的卡宾蛋白质足迹分析
J Am Soc Mass Spectrom. 2016 Mar;27(3):552-5. doi: 10.1007/s13361-015-1313-9. Epub 2015 Dec 17.
2
Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry.蛋白质快速光化学氧化与质谱联用
Protein Pept Lett. 2019;26(1):27-34. doi: 10.2174/0929866526666181128124554.
3
Mass Spectrometry-Based Fast Photochemical Oxidation of Proteins (FPOP) for Higher Order Structure Characterization.基于质谱的快速光化学氧化蛋白质（FPOP）用于高级结构表征。
Acc Chem Res. 2018 Mar 20;51(3):736-744. doi: 10.1021/acs.accounts.7b00593. Epub 2018 Feb 16.
4
Fast photochemical oxidation of proteins for comparing structures of protein-ligand complexes: the calmodulin-peptide model system.快速光化学氧化蛋白质以比较蛋白质-配体复合物的结构：钙调蛋白-肽模型系统。
Anal Chem. 2011 Jan 1;83(1):311-8. doi: 10.1021/ac102426d. Epub 2010 Dec 13.
5
Implementing fast photochemical oxidation of proteins (FPOP) as a footprinting approach to solve diverse problems in structural biology.将快速光化学氧化蛋白质（FPOP）作为一种足迹法用于解决结构生物学中的各种问题。
Methods. 2018 Jul 15;144:94-103. doi: 10.1016/j.ymeth.2018.05.016. Epub 2018 May 23.
6
A Fast Photochemical Oxidation of Proteins (FPOP) platform for free-radical reactions: the carbonate radical anion with peptides and proteins.一种用于自由基反应的快速光化学氧化蛋白质（FPOP）平台：碳酸根自由基阴离子与肽和蛋白质。
Free Radic Biol Med. 2019 Feb 1;131:126-132. doi: 10.1016/j.freeradbiomed.2018.11.031. Epub 2018 Nov 28.
7
New protein footprinting: fast photochemical iodination combined with top-down and bottom-up mass spectrometry.新型蛋白质足迹分析技术：快速光化学碘标记与自上而下和自下而上质谱相结合。
J Am Soc Mass Spectrom. 2012 Aug;23(8):1306-18. doi: 10.1007/s13361-012-0403-1. Epub 2012 Jun 6.
8
Characterization of ELISA Antibody-Antigen Interaction using Footprinting-Mass Spectrometry and Negative Staining Transmission Electron Microscopy.利用足迹质谱法和负染透射电子显微镜技术对 ELISA 抗体-抗原相互作用进行表征。
J Am Soc Mass Spectrom. 2018 May;29(5):961-971. doi: 10.1007/s13361-017-1883-9. Epub 2018 Mar 6.
9
Protein higher-order-structure determination by fast photochemical oxidation of proteins and mass spectrometry analysis.通过快速光化学氧化蛋白质和质谱分析来确定蛋白质的高级结构。
Nat Protoc. 2020 Dec;15(12):3942-3970. doi: 10.1038/s41596-020-0396-3. Epub 2020 Nov 9.
10
Variation in FPOP Measurements Is Primarily Caused by Poor Peptide Signal Intensity.FPOP 测量值的变化主要是由于肽信号强度差引起的。
J Am Soc Mass Spectrom. 2018 Sep;29(9):1901-1907. doi: 10.1007/s13361-018-1994-y. Epub 2018 Jun 25.
引用本文的文献
1
Mass Spectrometry-Based Protein Footprinting for Protein Structure Characterization.基于质谱的蛋白质足迹分析用于蛋白质结构表征
Acc Chem Res. 2025 Jan 21;58(2):165-176. doi: 10.1021/acs.accounts.4c00545. Epub 2025 Jan 5.
2
Characterization of Higher Order Structural Changes of a Thermally Stressed Monoclonal Antibody via Mass Spectrometry Footprinting and Other Biophysical Approaches.通过质谱指纹图谱和其他生物物理方法研究热应激单克隆抗体的高级结构变化特征。
Anal Chem. 2023 Nov 21;95(46):16840-16849. doi: 10.1021/acs.analchem.3c02422. Epub 2023 Nov 7.
3
Workflow for Validating Specific Amino Acid Footprinting Reagents for Protein Higher Order Structure Elucidation.蛋白质高级结构解析中特定氨基酸标记试剂验证的工作流程。
Anal Chem. 2023 Jul 4;95(26):10119-10126. doi: 10.1021/acs.analchem.3c01919. Epub 2023 Jun 23.
4
Advances in Mass Spectrometry on Membrane Proteins.膜蛋白质谱分析的进展
Membranes (Basel). 2023 Apr 24;13(5):457. doi: 10.3390/membranes13050457.
5
Structural mass spectrometry approaches to understand multidrug efflux systems.结构质谱方法研究多药外排系统。
Essays Biochem. 2023 Mar 29;67(2):255-267. doi: 10.1042/EBC20220190.
6
Using mass spectrometry-based methods to understand amyloid formation and inhibition of alpha-synuclein and amyloid beta.利用基于质谱的方法来了解α-突触核蛋白和淀粉样β的淀粉样形成和抑制。
Mass Spectrom Rev. 2024 Jul-Aug;43(4):782-825. doi: 10.1002/mas.21814. Epub 2022 Oct 12.
7
Top-Down Detection of Oxidative Protein Footprinting by Collision-Induced Dissociation, Electron-Transfer Dissociation, and Electron-Capture Dissociation.自上而下通过碰撞诱导解离、电子转移解离和电子俘获解离检测氧化蛋白质足迹。
Anal Chem. 2022 Jul 19;94(28):9993-10002. doi: 10.1021/acs.analchem.1c05476. Epub 2022 Jul 7.
8
Advances in mass spectrometry-based footprinting of membrane proteins.基于质谱的膜蛋白足迹分析技术的进展。
Proteomics. 2022 Apr;22(8):e2100222. doi: 10.1002/pmic.202100222.
9
Carbocation Footprinting of Soluble and Transmembrane Proteins.可溶性蛋白和跨膜蛋白的碳正离子足迹分析
Anal Chem. 2021 Oct 5;93(39):13101-13105. doi: 10.1021/acs.analchem.1c03274. Epub 2021 Sep 24.
10
Flash Oxidation (FOX) System: A Novel Laser-Free Fast Photochemical Oxidation Protein Footprinting Platform.闪氧（FOX）系统：一种新型的无激光快速光化学生物印迹平台。
J Am Soc Mass Spectrom. 2021 Jul 7;32(7):1601-1609. doi: 10.1021/jasms.0c00471. Epub 2021 Apr 19.
本文引用的文献
1
Solvent mimicry with methylene carbene to probe protein topography.用亚甲基卡宾进行溶剂模拟以探测蛋白质拓扑结构。
Anal Chem. 2015 Oct 6;87(19):10080-7. doi: 10.1021/acs.analchem.5b02724. Epub 2015 Sep 30.
2
Advances in radical probe mass spectrometry for protein footprinting in chemical biology applications.在化学生物学应用中，用于蛋白质足迹探测的激进探针质谱技术的进展。
Chem Soc Rev. 2014 May 21;43(10):3244-58. doi: 10.1039/c3cs60432b. Epub 2014 Mar 4.
3
Fast photochemical oxidation of proteins and mass spectrometry follow submillisecond protein folding at the amino-acid level.快速光化学氧化蛋白质和质谱法在氨基酸水平上跟踪亚毫秒级蛋白质折叠。
J Am Chem Soc. 2012 Nov 14;134(45):18724-31. doi: 10.1021/ja307606f. Epub 2012 Nov 1.
4
New protein footprinting: fast photochemical iodination combined with top-down and bottom-up mass spectrometry.新型蛋白质足迹分析技术：快速光化学碘标记与自上而下和自下而上质谱相结合。
J Am Soc Mass Spectrom. 2012 Aug;23(8):1306-18. doi: 10.1007/s13361-012-0403-1. Epub 2012 Jun 6.
5
High-resolution mapping of carbene-based protein footprints.基于卡宾的蛋白质足迹的高分辨率图谱绘制。
Anal Chem. 2012 May 15;84(10):4411-8. doi: 10.1021/ac300120z. Epub 2012 Apr 25.
6
Mass spectrometry-based carboxyl footprinting of proteins: method evaluation.基于质谱的蛋白质羧基足迹分析：方法评估
Int J Mass Spectrom. 2012 Feb 15;312:78-86. doi: 10.1016/j.ijms.2011.07.015.
7
Probing protein surface with a solvent mimetic carbene coupled to detection by mass spectrometry.用与质谱检测偶联的模拟溶剂的卡宾探测蛋白质表面。
J Am Soc Mass Spectrom. 2012 Jan;23(1):30-42. doi: 10.1007/s13361-011-0266-x. Epub 2011 Oct 18.
8
Fast photochemical oxidation of proteins for epitope mapping.快速光化学氧化蛋白质进行表位作图。
Anal Chem. 2011 Oct 15;83(20):7657-61. doi: 10.1021/ac2007366. Epub 2011 Sep 21.
9
Mass spectrometry of laser-initiated carbene reactions for protein topographic analysis.激光引发卡宾反应的质谱分析用于蛋白质形貌分析。
Anal Chem. 2011 Apr 15;83(8):2913-20. doi: 10.1021/ac102655f. Epub 2011 Mar 22.
10
Fast photochemical oxidation of proteins for comparing structures of protein-ligand complexes: the calmodulin-peptide model system.快速光化学氧化蛋白质以比较蛋白质-配体复合物的结构：钙调蛋白-肽模型系统。
Anal Chem. 2011 Jan 1;83(1):311-8. doi: 10.1021/ac102426d. Epub 2010 Dec 13.
Zotero 插件