Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran.
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
J Biomed Mater Res B Appl Biomater. 2018 Jul;106(5):1759-1769. doi: 10.1002/jbm.b.33980. Epub 2017 Sep 13.
Removal of leukocytes from blood products is the most effective means for elimination of undesirable side effects and prevention of possible reactions in recipients. Micro-fibrous mats are currently used for removal of leukocytes from blood. In this study, samples of electrospun nano-fibrous mats were produced. The performance of the produced electrospun nano-fibrous mats as means of leukocytes removal from fresh whole blood was both evaluated and compared with that of commercially available micro-fibrous mats. In order to produce the samples, polyacrylonitrile (PAN) nano-fibrous mats were made under different electrospinning conditions. Mean fiber diameter, pore characterization and surface roughness of the PAN nano-fibrous mats were determined using image processing technique. In order to evaluate the surface tension of the fabricated mats, water contact angle was measured. The leukocyte removal performance, erythrocytes recovery percent and hemolysis rate of the nano-fibrous mats were compared. The effectiveness of nano-fibrous mats in removing leukocyte was established using both scanning electron microscope and optical microscope. Results showed that for given weight, the fabricated nano-fibrous mats were not only more efficient but also more cost-effective than their commercial counterparts. Results confirmed that changes in mean fiber diameter, the number of layer and weight of each layer in the absence of any chemical reaction or physical surface modification, the fabricated nano-fibrous mats were able to remove 5-log of leukocytes. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1759-1769, 2018.
从血液制品中去除白细胞是消除受者不良副作用和预防可能反应的最有效手段。微纤维垫目前用于去除血液中的白细胞。在这项研究中，生产了电纺纳米纤维垫的样本。评估了所生产的电纺纳米纤维垫作为从新鲜全血中去除白细胞的手段的性能，并与市售的微纤维垫进行了比较。为了生产样品，在不同的静电纺丝条件下制造了聚丙烯腈（PAN）纳米纤维垫。使用图像处理技术确定了 PAN 纳米纤维垫的平均纤维直径、孔特征和表面粗糙度。为了评估所制造的垫子的表面张力，测量了水接触角。比较了纳米纤维垫的白细胞去除性能、红细胞回收率和溶血率。使用扫描电子显微镜和光学显微镜评估了纳米纤维垫去除白细胞的效果。结果表明，对于给定的重量，所制造的纳米纤维垫不仅更有效，而且比其商业对应物更具成本效益。结果证实，在没有任何化学反应或物理表面改性的情况下，改变平均纤维直径、层数和每层的重量，所制造的纳米纤维垫能够去除 5 对数的白细胞。©2017 威利父子公司。生物医学材料研究杂志 B：应用生物材料，106B:1759-1769，2018 年。
