化学生物学方法研究翻译后修饰。
Chemical biology approaches for studying posttranslational modifications.
机构信息
a Department of Chemistry , Korea Advanced Institute of Science and Technology , 291 Daehak-ro, Yuseong-gu , Daejeon , Republic of Korea.
出版信息
RNA Biol. 2018;15(4-5):427-440. doi: 10.1080/15476286.2017.1360468. Epub 2017 Sep 21.
Abstract
Posttranslational modification (PTM) is a key mechanism for regulating diverse protein functions, and thus critically affects many essential biological processes. Critical for systematic study of the effects of PTMs is the ability to obtain recombinant proteins with defined and homogenous modifications. To this end, various synthetic and chemical biology approaches, including genetic code expansion and protein chemical modification methods, have been developed. These methods have proven effective for generating site-specific authentic modifications or structural mimics, and have demonstrated their value for in vitro and in vivo functional studies of diverse PTMs. This review will discuss recent advances in chemical biology strategies and their application to various PTM studies.
摘要
翻译
翻译后修饰（PTM）是调节多种蛋白质功能的关键机制，因此对许多重要的生物学过程至关重要。系统研究 PTM 影响的关键是获得具有定义和均一修饰的重组蛋白的能力。为此，已经开发了各种合成和化学生物学方法，包括遗传密码扩展和蛋白质化学修饰方法。这些方法已被证明可有效地生成具有特异性的真实修饰或结构类似物，并已证明它们在各种 PTM 的体外和体内功能研究中的价值。本综述将讨论化学生物学策略的最新进展及其在各种 PTM 研究中的应用。
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