Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Sensors (Basel). 2017 Sep 13;17(9):1986. doi: 10.3390/s17091986.
The enrichment of low-abundance proteins in complex biological samples plays an important role in clinical diagnostics and biomedical research. This work reports a novel one-step method for the synthesis of aptamer-modified graphene oxide (GO/Apt) nanocomposites, without introducing the use of gold, for the rapid and specific separation and enrichment of human α-thrombin from buffer solutions with highly concentrated interferences. The obtained GO/Apt nanocomposites had remarkable aptamer immobilization, up to 44.8 nmol/mg. Furthermore, GO/Apt nanocomposites exhibited significant specific enrichment efficiency for human α-thrombin (>90%), even under the presence of 3000-fold interference proteins, which was better than the performance of other nanomaterials. Finally, the GO/Apt nanocomposites were applied in the specific capturing of human α-thrombin in highly concentrated human plasma solutions with negligible nonspecific binding of other proteins, which demonstrated their prospects in rare protein analysis and biosensing applications.
在复杂的生物样本中富集低丰度蛋白质在临床诊断和生物医学研究中起着重要作用。本工作报道了一种用于合成适体修饰氧化石墨烯（GO/Apt）纳米复合材料的新一步法，无需引入金，可快速、特异性地从缓冲溶液中分离和富集高浓度干扰物的人α-凝血酶。所得的 GO/Apt 纳米复合材料具有显著的适体固定化能力，高达 44.8 nmol/mg。此外，GO/Apt 纳米复合材料对人α-凝血酶表现出显著的特异性富集效率（>90%），即使存在 3000 倍的干扰蛋白，也优于其他纳米材料的性能。最后，GO/Apt 纳米复合材料在高浓度人血浆溶液中特异性捕获人α-凝血酶，对其他蛋白质的非特异性结合可忽略不计，这表明其在稀有蛋白质分析和生物传感应用中具有广阔的前景。
