• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

C/EBPα 和 SUMO 修饰在肺发育中的功能作用。

Functional roles of C/EBPα and SUMO‑modification in lung development.

机构信息

Dean's Office, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China.

Department of Pediatrics, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China.

出版信息

Int J Mol Med. 2017 Oct;40(4):1037-1046. doi: 10.3892/ijmm.2017.3111. Epub 2017 Aug 29.

DOI:10.3892/ijmm.2017.3111
PMID:28902364
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5593452/
Abstract

CCAAT enhancer binding protein alpha (C/EBPα) is a transcription factor regulating the core aspects of cell growth and differentiation. The present study investigated the level and functional role of C/EBPα during the development of the rat lung. C/EBPα protein exhibits a dynamic expression pattern. The correlation between the expression of C/EBPα protein and the content of glycogen during lung maturation was analyzed to understand the function of C/EBPα in lung differentiation. The high expression of C/EBPα coincides with the reduction of glycogen in the fetal lung. In addition, the authors identified that changes in the level of C/EBPα are associated with the secretion of pulmonary surfactant. C/EBPα is modified by small ubiquitin-related modifier (SUMO) post-translationally. The results of double immunofluorescence staining and immunoprecipitation demonstrated that SUMO-modified C/EBPα was present in the lung. The sumoylated C/EBPα gradually decreased during lung differentiation and was negatively correlated with pulmonary surfactant secretion, thereby suggesting that the SUMO modification may participate in C/EBPα-mediated lung growth and differentiation. These results indicated that C/EBPα played a role in lung development and provided the insight into the mechanism underlying SUMO-modification.

摘要

CCAAT 增强子结合蛋白α（C/EBPα）是一种转录因子，调节细胞生长和分化的核心方面。本研究调查了 C/EBPα 在大鼠肺发育过程中的水平和功能作用。C/EBPα 蛋白表现出动态表达模式。分析 C/EBPα 蛋白表达与肺成熟过程中糖原含量之间的相关性，以了解 C/EBPα 在肺分化中的功能。C/EBPα 的高表达与胎儿肺中糖原的减少相一致。此外，作者发现 C/EBPα 水平的变化与肺表面活性物质的分泌有关。C/EBPα 被小泛素相关修饰物（SUMO）进行翻译后修饰。双重免疫荧光染色和免疫沉淀的结果表明 SUMO 修饰的 C/EBPα 存在于肺中。在肺分化过程中，SUMO 修饰的 C/EBPα 逐渐减少，与肺表面活性物质的分泌呈负相关，提示 SUMO 修饰可能参与 C/EBPα 介导的肺生长和分化。这些结果表明 C/EBPα 在肺发育中起作用，并为 SUMO 修饰的机制提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/f66af0812c6b/IJMM-40-04-1037-g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/d889e4554d54/IJMM-40-04-1037-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/f716e25ce6df/IJMM-40-04-1037-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/c2e6b13568af/IJMM-40-04-1037-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/6fd53dd76fac/IJMM-40-04-1037-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/33f778992da8/IJMM-40-04-1037-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/cc017de25338/IJMM-40-04-1037-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/cabf636333ee/IJMM-40-04-1037-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/9ba9b5cca0d8/IJMM-40-04-1037-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/c566c873ee1d/IJMM-40-04-1037-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/f66af0812c6b/IJMM-40-04-1037-g09.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/d889e4554d54/IJMM-40-04-1037-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/f716e25ce6df/IJMM-40-04-1037-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/c2e6b13568af/IJMM-40-04-1037-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/6fd53dd76fac/IJMM-40-04-1037-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/33f778992da8/IJMM-40-04-1037-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/cc017de25338/IJMM-40-04-1037-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/cabf636333ee/IJMM-40-04-1037-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/9ba9b5cca0d8/IJMM-40-04-1037-g07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/c566c873ee1d/IJMM-40-04-1037-g08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3c44/5593452/f66af0812c6b/IJMM-40-04-1037-g09.jpg

相似文献

1
Functional roles of C/EBPα and SUMO‑modification in lung development.C/EBPα 和 SUMO 修饰在肺发育中的功能作用。
Int J Mol Med. 2017 Oct;40(4):1037-1046. doi: 10.3892/ijmm.2017.3111. Epub 2017 Aug 29.
2
Sumoylation of CCAAT-enhancer-binding protein α inhibits lung differentiation in Bronchopulmonary Dysplasia model rats.CCAAT 增强子结合蛋白α的 SUMOylation 抑制支气管肺发育不良模型大鼠的肺分化。
J Cell Mol Med. 2020 Jun;24(12):7067-7071. doi: 10.1111/jcmm.15310. Epub 2020 May 4.
3
Sumoylation of CCAAT/enhancer-binding protein alpha and its functional roles in hepatocyte differentiation.CCAAT/增强子结合蛋白α的类泛素化修饰及其在肝细胞分化中的功能作用
J Biol Chem. 2006 Aug 4;281(31):21629-21639. doi: 10.1074/jbc.M600852200. Epub 2006 May 30.
4
A synergy control motif within the attenuator domain of CCAAT/enhancer-binding protein alpha inhibits transcriptional synergy through its PIASy-enhanced modification by SUMO-1 or SUMO-3.CCAAT/增强子结合蛋白α的衰减域内的协同控制基序通过其由SUMO-1或SUMO-3进行的PIASy增强修饰来抑制转录协同作用。
J Biol Chem. 2003 Mar 14;278(11):9134-41. doi: 10.1074/jbc.M210440200. Epub 2003 Jan 2.
5
C/EBPalpha is required for lung maturation at birth.出生时肺成熟需要C/EBPα。
Development. 2006 Mar;133(6):1155-64. doi: 10.1242/dev.02273. Epub 2006 Feb 8.
6
Lipogenesis in fetal rat lung: importance of C/EBPalpha, SREBP-1c, and stearoyl-CoA desaturase.胎鼠肺中的脂肪生成：C/EBPα、SREBP-1c和硬脂酰辅酶A去饱和酶的重要性。
Am J Respir Cell Mol Biol. 2004 Feb;30(2):174-83. doi: 10.1165/rcmb.2003-0235OC. Epub 2003 Aug 1.
7
Ectopic expression of C/EBPalpha in the lung epithelium disrupts late lung development.肺上皮细胞中C/EBPα的异位表达会破坏肺的后期发育。
Am J Physiol Lung Cell Mol Physiol. 2006 Oct;291(4):L683-93. doi: 10.1152/ajplung.00497.2005. Epub 2006 May 12.
8
Airway epithelial cell differentiation during lung organogenesis requires C/EBPα and C/EBPβ.肺器官发生过程中气道上皮细胞的分化需要 C/EBPα 和 C/EBPβ。
Dev Dyn. 2012 May;241(5):911-23. doi: 10.1002/dvdy.23773. Epub 2012 Mar 23.
9
Sumoylation of CCAAT/enhancer-binding protein α is implicated in hematopoietic stem/progenitor cell development through regulating runx1 in zebrafish.CCAAT/增强子结合蛋白α的类泛素化修饰通过调控斑马鱼中的runx1参与造血干/祖细胞发育。
Sci Rep. 2015 Mar 11;5:9011. doi: 10.1038/srep09011.
10
[The 161th lysine of C/EBPα in alveolar type II epithelial cells is modified by small ubiquitin-related modification].[肺泡II型上皮细胞中C/EBPα的第161位赖氨酸被小泛素相关修饰修饰]
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2019 Feb;35(2):158-161.

引用本文的文献

1
The Role of SUMO1 Modification of SOX9 in Cartilage Development Stimulated by Zinc Ions in Mice.SUMO1修饰的SOX9在锌离子刺激的小鼠软骨发育中的作用
Organogenesis. 2025 Dec;21(1):2460269. doi: 10.1080/15476278.2025.2460269. Epub 2025 Feb 4.
2
Epigenetic modifications in the development of bronchopulmonary dysplasia: a review.支气管肺发育不良的发生发展中的表观遗传修饰：综述。
Pediatr Res. 2024 Aug;96(3):632-642. doi: 10.1038/s41390-024-03167-7. Epub 2024 Apr 3.
3
The emerging roles of SUMOylation in pulmonary diseases.SUMOylation 在肺部疾病中的新兴作用。

本文引用的文献

1
Lung development.肺发育
Semin Pediatr Surg. 2015 Aug;24(4):152-5. doi: 10.1053/j.sempedsurg.2015.01.011. Epub 2015 Feb 2.
2
Regulation of pulmonary surfactant synthesis in fetal rat type II alveolar epithelial cells by microRNA-26a.微小RNA-26a对胎鼠II型肺泡上皮细胞肺表面活性物质合成的调控
Pediatr Pulmonol. 2014 Sep;49(9):863-72. doi: 10.1002/ppul.22975. Epub 2014 Jan 3.
3
HDAC3 interacts with sumoylated C/EBPα to negatively regulate the LXRα expression in rat hepatocytes.HDAC3 与 SUMO 化的 C/EBPα 相互作用，负调控大鼠肝细胞中 LXRα 的表达。
Mol Med. 2023 Sep 5;29(1):119. doi: 10.1186/s10020-023-00719-1.
4
Protein posttranslational modifications in health and diseases: Functions, regulatory mechanisms, and therapeutic implications.健康与疾病中的蛋白质翻译后修饰：功能、调控机制及治疗意义
MedComm (2020). 2023 May 2;4(3):e261. doi: 10.1002/mco2.261. eCollection 2023 Jun.
5
Mechanism of Adipose-Derived Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying miR-21-5p in Hyperoxia-Induced Lung Injury.脂肪间充质干细胞来源的细胞外囊泡携带 miR-21-5p 在高氧诱导肺损伤中的作用机制。
Stem Cell Rev Rep. 2022 Mar;18(3):1007-1024. doi: 10.1007/s12015-021-10311-x. Epub 2021 Dec 9.
6
Sumoylation of CCAAT-enhancer-binding protein α inhibits lung differentiation in Bronchopulmonary Dysplasia model rats.CCAAT 增强子结合蛋白α的 SUMOylation 抑制支气管肺发育不良模型大鼠的肺分化。
J Cell Mol Med. 2020 Jun;24(12):7067-7071. doi: 10.1111/jcmm.15310. Epub 2020 May 4.
7
Presence of N-acetylgalactosamine/galactose residues on bronchioloalveolar cells during rat postnatal development.大鼠出生后发育过程中肺泡细胞表面 N-乙酰半乳糖胺/半乳糖残基的存在。
Eur J Histochem. 2019 Sep 11;63(3):3040. doi: 10.4081/ejh.2019.3040.
8
Effects of C/EBPα overexpression on alveolar epithelial type II cell proliferation, apoptosis and surfactant protein-C expression after exposure to hyperoxia.过表达 C/EBPα 对高氧暴露后肺泡上皮细胞 II 型增殖、凋亡和表面活性蛋白-C 表达的影响。
BMC Pulm Med. 2019 Aug 6;19(1):142. doi: 10.1186/s12890-019-0911-x.
9
SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells.SENP1 在肺发育中具有重要作用，并影响肺泡型 2 细胞的分化。
Int J Mol Med. 2019 Jan;43(1):371-381. doi: 10.3892/ijmm.2018.3964. Epub 2018 Oct 29.
10
[Dynamic expression and role of SUMO-modified C/EBPα in preterm rats with bronchopulmonary dysplasisa induced by hyperoxia exposure].[SUMO修饰的C/EBPα在高氧诱导的早产支气管肺发育不良大鼠中的动态表达及作用]
Zhongguo Dang Dai Er Ke Za Zhi. 2018 May;20(5):403-409. doi: 10.7499/j.issn.1008-8830.2018.05.013.
Mol Cell Endocrinol. 2013 Jul 15;374(1-2):35-45. doi: 10.1016/j.mce.2013.04.013. Epub 2013 Apr 29.
4
Asthma and COPD - The C/EBP Connection.哮喘与慢性阻塞性肺疾病——C/EBP 关联
Open Respir Med J. 2012;6:1-13. doi: 10.2174/1874306401206010001. Epub 2012 Apr 20.
5
Differential effects of sumoylation on the activities of CCAAT enhancer binding protein alpha (C/EBPα) p42 versus p30 may contribute in part, to aberrant C/EBPα activity in acute leukemias.SUMO化修饰对CCAAT增强子结合蛋白α（C/EBPα）p42和p30活性的不同影响可能在一定程度上导致了急性白血病中C/EBPα活性异常。
Hematol Rep. 2011 Jan 13;3(1):e5. doi: 10.4081/hr.2011.e5. Epub 2011 May 20.
6
The CCAAT/enhancer (C/EBP) family of basic-leucine zipper (bZIP) transcription factors is a multifaceted highly-regulated system for gene regulation.CCAAT/增强子结合蛋白（C/EBP）家族的碱性亮氨酸拉链（bZIP）转录因子是一个多方面的高度调控的基因调控系统。
Cytokine. 2011 Apr;54(1):6-19. doi: 10.1016/j.cyto.2010.12.019. Epub 2011 Jan 22.
7
The 'new' bronchopulmonary dysplasia: challenges and commentary.新型支气管肺发育不良：挑战与述评。
Semin Fetal Neonatal Med. 2009 Dec;14(6):345-57. doi: 10.1016/j.siny.2009.08.009. Epub 2009 Sep 10.
8
Lung-specific inactivation of CCAAT/enhancer binding protein alpha causes a pathological pattern characteristic of COPD.肺特异性敲除 CCAAT/增强子结合蛋白α导致具有 COPD 特征的病理性模式。
Eur Respir J. 2010 Jan;35(1):186-97. doi: 10.1183/09031936.00185008. Epub 2009 Jul 16.
9
Sumoylation and the function of CCAAT enhancer binding protein alpha (C/EBP alpha).小泛素样修饰以及CCAAT增强子结合蛋白α（C/EBPα）的功能
Blood Cells Mol Dis. 2008 Jul-Aug;41(1):77-81. doi: 10.1016/j.bcmd.2008.02.011. Epub 2008 Apr 10.
10
Target proteins of C/EBPalphap30 in AML: C/EBPalphap30 enhances sumoylation of C/EBPalphap42 via up-regulation of Ubc9.急性髓系白血病中C/EBPαp30的靶蛋白：C/EBPαp30通过上调Ubc9增强C/EBPαp42的SUMO化修饰。
Blood. 2007 Nov 1;110(9):3301-9. doi: 10.1182/blood-2007-01-071035. Epub 2007 Aug 1.