Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Environ Toxicol Pharmacol. 2017 Oct;55:202-207. doi: 10.1016/j.etap.2017.08.038. Epub 2017 Sep 1.
Trichloroethylene (TCE) is a chlorinated organic pollutant of groundwater with diverse toxic effects in animals and humans. Here, we investigated the ameliorative role of hesperidin, a citrus bioflavonoid on TCE-induced toxicity in Drosophila melanogaster. Four groups of D. melanogaster (50 flies/vial, with 5 vials/group) were exposed to ethanol (2.5%, control), HSP (400mg/10g diet), TCE (10μM/10g diet) and TCE (10μM/10g diet)+HSP (400mg/10g diet) respectively in the diet for 5days. Then, selected oxidative stress and antioxidant markers were evaluated. The results showed that TCE significantly increased the level of reactive oxygen species (ROS) and inhibited catalase, glutathione S-transferase and acetylcholinesterase (AChE) activities with concurrent depletion of total thiol level. However, co-administration of TCE and hesperidin mitigated TCE-induced depletion of antioxidants, and restored ROS level and AChE activity in the flies (p<0.05). Overall, hesperidin offered protective potency on TCE-induced oxidative stress in the flies via anti-oxidative mechanism.
三氯乙烯（TCE）是一种对地下水具有多种毒性作用的氯化有机污染物，在动物和人类中都有不同的毒性作用。在这里，我们研究了橙皮苷（一种柑橘类生物类黄酮）对黑腹果蝇 TCE 诱导毒性的改善作用。将四组黑腹果蝇（每组 50 只，每组 5 个瓶）分别暴露于乙醇（2.5%，对照）、HSP（400mg/10g 饮食）、TCE（10μM/10g 饮食）和 TCE（10μM/10g 饮食）+HSP（400mg/10g 饮食）饮食中 5 天。然后，评估了选定的氧化应激和抗氧化标志物。结果表明，TCE 显著增加了活性氧（ROS）的水平，并抑制了过氧化氢酶、谷胱甘肽 S-转移酶和乙酰胆碱酯酶（AChE）的活性，同时总巯基水平降低。然而，TCE 和橙皮苷的共同给药减轻了 TCE 诱导的抗氧化剂耗竭，并恢复了果蝇中的 ROS 水平和 AChE 活性（p<0.05）。总的来说，橙皮苷通过抗氧化机制为 TCE 诱导的氧化应激提供了保护作用。
