Department of Chemical Sciences, Ajayi Crowther University, Oyo, Nigeria.
Department of Biological Sciences, KolaDaisi University, Ibadan, Oyo State, Nigeria.
Comp Biochem Physiol C Toxicol Pharmacol. 2023 Jan;263:109500. doi: 10.1016/j.cbpc.2022.109500. Epub 2022 Nov 5.
Cadmium chloride (CdCl) is an important heavy metal widely regarded as an environmental contaminant. Hesperidin, a flavanone glycoside found in citrus fruits, has an established properties against free radicals, apoptosis, and inflammation. The present study investigated the protective actions of hesperidin on CdCl-induced oxidative damage and inflammation in Drosophila melanogaster. For 7 consecutive days via their diet regimen, the flies were exposed to CdCl alone (0.05 mM) or in combination with hesperidin (50 and 100 μM). Exposure to CdCl significantly (p < 0.05) increased mortality rate of flies, whereas the survived flies demonstrated significant oxidative toxicity from decreased activities of catalase and Glutathione S-transferase (GST) and Total Thiol (T-SH) and Non-Protein Thiols (NPSH) levels as well as accumulation of Nitric Oxide (NO (nitrite/nitrate)), protein carbonyl and Hydrogen Peroxide (HO). However, hesperidin-supplemented diet improved Acetylcholinesterase (AChE) activity, mitochondrial metabolic rate (cell viability), locomotor activity, and amelioration of oxidative damage and lipid peroxidation induced by CdCl. The hesperidin diet supplement boosted the antioxidant milieu and ameliorated the oxidative damage in the treated flies. Overall, the findings revealed that hesperidin improved antioxidative protective capacity in Drosophila melanogaster model of CdCl-induced toxicity. This suggests hesperidin as a potential therapeutic agent against oxidative stress disorders due to exposure to CdCl and or related toxicants.
氯化镉（CdCl）是一种重要的重金属，被广泛认为是环境污染物。橙皮苷是柑橘类水果中发现的一种黄烷酮糖苷，具有清除自由基、抗细胞凋亡和抗炎的特性。本研究探讨了橙皮苷对黑腹果蝇中 CdCl 诱导的氧化损伤和炎症的保护作用。通过饮食方案，连续 7 天，使果蝇单独暴露于 CdCl（0.05mM）或与橙皮苷（50 和 100μM）联合暴露。暴露于 CdCl 显著增加了果蝇的死亡率（p<0.05），而存活的果蝇表现出明显的氧化毒性，表现为过氧化氢酶和谷胱甘肽 S-转移酶（GST）以及总巯基（T-SH）和非蛋白巯基（NPSH）水平的活性降低，以及一氧化氮（NO（亚硝酸盐/硝酸盐））、蛋白质羰基和过氧化氢（HO）的积累。然而，橙皮苷补充饮食改善了乙酰胆碱酯酶（AChE）活性、线粒体代谢率（细胞活力）、运动活性以及 CdCl 诱导的氧化损伤和脂质过氧化的改善。橙皮苷饮食补充剂增强了抗氧化环境，改善了处理果蝇的氧化损伤。总的来说，这些发现表明橙皮苷提高了黑腹果蝇模型中 CdCl 诱导毒性的抗氧化保护能力。这表明橙皮苷可能是一种对抗因接触 CdCl 和/或相关毒物引起的氧化应激障碍的潜在治疗剂。
