来自大肠杆菌的D-半乳糖/D-葡萄糖结合蛋白作为一种非消耗性葡萄糖可植入荧光生物传感器的探针。
D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor.
作者信息
Scognamiglio Viviana, Aurilia Vincenzo, Cennamo Nunzio, Ringhieri Paola, Iozzino Luisa, Tartaglia Micaela, Staiano Maria, Ruggiero Giuseppe, Orlando Pierangelo, Labella Tullio, Zeni Luigi, Vitale Annalisa, D'Auria Sabato
机构信息
Laboratory for Molecular Sensing, IBP, CNR, Via Pietro Castellino, 111 80131 Naples, Italy.
Department of Information Engineering, Seconda Università di Napoli, Italy.
出版信息
Sensors (Basel). 2007 Oct 24;7(10):2484-2491. doi: 10.3390/s7102484.
D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care.
来自大肠杆菌的D-半乳糖/D-葡萄糖结合蛋白（GGBP）是一种以高亲和力结合葡萄糖的单体。GGBP的蛋白质结构由两个主要结构域组成，通过一个铰链区相连，形成糖结合位点。在这项工作中，我们表明，位于氨基酸位置182的GGBP突变形式可作为一种探针，用于开发一种非消耗性分析物荧光生物传感器，以监测糖尿病医疗保健中的葡萄糖水平。
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