Calvi Jessica L, Chen Frances R, Benson Victoria Brann, Brindle Eleanor, Bristow Matt, De Alpana, Entringer Sonja, Findlay Helen, Heim Christine, Hodges Eric A, Klawitter Heiko, Lupien Sonia, Rus Holly M, Tiemensma Jitske, Verlezza Silvanna, Walker Claire-Dominique, Granger Douglas A
Salivary Bioscience Laboratory, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Department of Criminal Justice and Criminology, Georgia State University, Atlanta, GA, 30302, USA.
BMC Res Notes. 2017 Sep 13;10(1):479. doi: 10.1186/s13104-017-2805-4.
Hundreds of scientific publications are produced annually that involve the measurement of cortisol in saliva. Intra- and inter-laboratory variation in salivary cortisol results has the potential to contribute to cross-study inconsistencies in findings, and the perception that salivary cortisol results are unreliable. This study rigorously estimates sources of measurement variability in the assay of salivary cortisol within and between established international academic-based laboratories that specialize in saliva analyses. One hundred young adults (Mean age: 23.10 years; 62 females) donated 2 mL of whole saliva by passive drool. Each sample was split into multiple- 100 µL aliquots and immediately frozen. One aliquot of each of the 100 participants' saliva was transported to academic laboratories (N = 9) in the United States, Canada, UK, and Germany and assayed for cortisol by the same commercially available immunoassay.
1.76% of the variance in salivary cortisol levels was attributable to differences between duplicate assays of the same sample within laboratories, 7.93% of the variance was associated with differences between laboratories, and 90.31% to differences between samples. In established-qualified laboratories, measurement error of salivary cortisol is minimal, and inter-laboratory differences in measurement are unlikely to have a major influence on the determined values.
每年有数百篇科学出版物涉及唾液中皮质醇的测量。唾液皮质醇检测结果在实验室内和实验室间的差异有可能导致不同研究结果出现不一致的情况，进而让人觉得唾液皮质醇检测结果不可靠。本研究严格评估了在专门从事唾液分析的国际知名学术实验室内部和之间进行唾液皮质醇检测时测量变异性的来源。100名年轻人（平均年龄：23.10岁；62名女性）通过被动流涎捐献了2毫升全唾液。每个样本被分成多个100微升的等分试样并立即冷冻。100名参与者的唾液各取一份等分试样被运送到美国、加拿大、英国和德国的学术实验室（N = 9），并使用相同的市售免疫分析法检测皮质醇。
唾液皮质醇水平差异的1.76%可归因于实验室内同一样本重复检测之间的差异，7.93%的差异与实验室间差异有关，90.31%的差异与样本间差异有关。在资质合格的实验室中，唾液皮质醇的测量误差极小，实验室间的测量差异不太可能对测定值产生重大影响。
