Demeulemeester H, Vandesande F, Orban G A, Brandon C, Vanderhaeghen J J
Labortory of Neuroendocrinology and Immunological Biotechnology, Zoological Institute, Leuven, Belgium.
J Neurosci. 1988 Mar;8(3):988-1000. doi: 10.1523/JNEUROSCI.08-03-00988.1988.
Antibodies against neuropeptides and against a vitamin D-dependent calcium-binding protein (CaBP) label small cells with nonpyramidal-like morphology in the cat visual cortex (areas 17, 18, and 19). Since GABAergic cells are interneurons, a double-staining procedure was used to test for the coexistence of cholecystokinin (CCK), somatostatin (SRIF), neuropeptide Y (NPY), corticotropin-releasing factor (CRF), vasoactive intestinal polypeptide (VIP), and CaBP with glutamic acid decarboxylase (GAD). Our results show that CRF and VIP do not coexist with GAD, while the 3 other peptides and CaBP do. Hence GAD-positive cells can be subdivided into 4 broad groups: (1) cells that are only GAD-positive, (2) cells that are GAD- and CaBP-positive, (3) GAD-positive neurons also containing CCK, and (4) GAD-positive cells that also contain SRIF. A small subset of class 2 also contains SRIF and most cells of class 4 also contain NPY. The 4 classes of GAD-positive cells differ in laminar position: class 1 predominates in layers IV and V, classes 2 and 3 in the upper laminae (II and III), and class 4 in the deepest layer (VI).
针对神经肽以及一种维生素D依赖性钙结合蛋白（CaBP）的抗体，可标记猫视觉皮层（17、18和19区）中具有非锥体样形态的小细胞。由于γ-氨基丁酸能细胞是中间神经元，因此采用双重染色程序来检测胆囊收缩素（CCK）、生长抑素（SRIF）、神经肽Y（NPY）、促肾上腺皮质激素释放因子（CRF）、血管活性肠多肽（VIP）和CaBP与谷氨酸脱羧酶（GAD）是否共存。我们的结果表明，CRF和VIP与GAD不共存，而其他3种肽和CaBP与GAD共存。因此，GAD阳性细胞可分为4大类：（1）仅GAD阳性的细胞，（2）GAD和CaBP阳性的细胞，（3）同时含有CCK的GAD阳性神经元，以及（4）同时含有SRIF的GAD阳性细胞。第2类的一小部分也含有SRIF，第4类的大多数细胞也含有NPY。这4类GAD阳性细胞在层位上有所不同：第1类在IV层和V层占主导，第2类和第3类在上层（II层和III层），第4类在最深层（VI层）。
