Laboratorio de Investigación Microbiológica, UIMyT, Hospital Juárez de México, Av. Instituto Politécnico Nacional # 5160 Col. Magdalena de las Salinas, Ciudad de México, México, C.P., 07650, Mexico.
Departamento de Medicina Genómica y Toxicología Ambiental. Instituto de Investigaciones Biomédicas Edificio C, 2° Piso, Lab C-205. Circuito Mario de la Cueva s/n, Coyoacán, Ciudad Universitaria, 04510, Ciudad de México, Mexico.
Sci Rep. 2017 Sep 13;7(1):11479. doi: 10.1038/s41598-017-11818-5.
The essential oils of Citrus sinensis and Citrus latifolia showed antimycotic activity against Candida spp. isolated from the oral cavity; they are neither mutagenic on the Ames test nor cytotoxic. Their main components are R-(+)-limonene, β-thujene, α-myrcene and γ-terpinene. The aim of this work was to evaluate their antimutagenic and antioxidant capacities. Antimutagenic properties were evaluated against MNNG and ENNG on S. typhimurium TA100; against 2AA on strain TA98 and in front of 4NQO and NOR on strain TA102. Both were antimutagenic against MNNG (p < 0.001) but only C. latifolia was antimutagenic against ENNG (p < 0.001). Both presented antimutagenic activity against 2AA (p < 0.001). They were antioxidant against the ROS-generating compound 4NQO (p < 0.001) and the antibiotic NOR (p < 0.001). In the antioxidant evaluation, the activity in DPPH assay was in a range of 6-23% for C. sinensis and of 22-71% for C. latifolia. Both were antioxidant compared with BHT in β-carotene bleaching assay and were able to decreased apoptosis in HaCat cells stimulated with HO. The levels of intracellular superoxide ion were lower in the presence of both oils. In conclusion, the essential oils of C. sinensis and C. latifolia are antimutagenic against at least three types of mutagens and have antioxidants properties.
甜橙和莱檬的精油对口腔分离的念珠菌属有抗真菌活性；它们在 Ames 试验中既没有致突变性，也没有细胞毒性。它们的主要成分是 R-(+)-柠檬烯、β-蒎烯、α-松油烯和γ-萜品烯。本研究旨在评估它们的抗突变和抗氧化能力。抗突变特性在 S. typhimurium TA100 中用 MNNG 和 ENNG 进行评估；在 TA98 菌株中用 2AA 进行评估，在 TA102 菌株中用 4NQO 和 NOR 进行评估。两者对 MNNG 均具有抗突变作用（p<0.001），但只有莱檬精油对 ENNG 具有抗突变作用（p<0.001）。两者对 2AA 均具有抗突变活性（p<0.001）。它们对产生 ROS 的化合物 4NQO（p<0.001）和抗生素 NOR（p<0.001）均具有抗氧化作用。在抗氧化评估中，DPPH 测定法中甜橙精油的活性范围为 6-23%，莱檬精油的活性范围为 22-71%。在β-胡萝卜素漂白测定法中，与 BHT 相比，两者均具有抗氧化作用，并且能够降低 HO 刺激的 HaCat 细胞凋亡。在两种油存在的情况下，细胞内超氧阴离子的水平较低。综上所述，甜橙和莱檬的精油对至少三种类型的诱变剂具有抗突变作用，并且具有抗氧化特性。
Zotero 插件
