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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Petronijevic T, Rogers W P

Department of Plant Physiology, Waite Agricultural Research Institute, South Australia.

Comp Biochem Physiol A Comp Physiol. 1987;88(2):207-12. doi: 10.1016/0300-9629(87)90471-3.

H2CO3, the host's signal which induced (less than 25 min) the Ca2+-dependent development of the first parasitic stage of Haemonchus contortus, produced a diphasic change in the pHi (less than 30 min), measured with 5,5-dimethyl-2,4-oxazolidinedione, in the infective stage. The intensity of the diphasic change was related to the [H2CO3] and so to the efficiency of the stimulus for development. 2. MES and HEPES buffers induced a rapid rise in pHi of infective stages in step with pHo. Ortho-and pyrophosphate induced greater changes in pHi. Such buffers did not initiate development. 3. These and other results suggest that the stimulus for development, H2CO3, induced an energy-dependent Ca2+/H+ exchange mediated by mitochondria of the infective stage, which initiated development of the parasitic stage.

宿主信号H2CO3可诱导捻转血矛线虫（Haemonchus contortus）第一个寄生阶段的钙离子依赖性发育（诱导时间小于25分钟），在用5,5 - 二甲基 - 2,4 - 恶唑烷二酮测量感染阶段的细胞内pH值（pHi）时，H2CO3会在30分钟内引起pHi的双相变化。双相变化的强度与[H2CO3]相关，因此也与发育刺激的效率相关。2. MES和HEPES缓冲液会使感染阶段的pHi随细胞外pH值（pHo）同步快速升高。正磷酸盐和焦磷酸盐会引起pHi的更大变化。这些缓冲液不会引发发育。3. 这些及其他结果表明，发育刺激物H2CO3会诱导感染阶段线粒体介导的能量依赖性Ca2+/H+交换，从而启动寄生阶段的发育。

Petronijevic T, Rogers W P

Department of Plant Physiology, Waite Agricultural Research Institute, South Australia.

Comp Biochem Physiol A Comp Physiol. 1987;88(2):207-12. doi: 10.1016/0300-9629(87)90471-3.

H2CO3, the host's signal which induced (less than 25 min) the Ca2+-dependent development of the first parasitic stage of Haemonchus contortus, produced a diphasic change in the pHi (less than 30 min), measured with 5,5-dimethyl-2,4-oxazolidinedione, in the infective stage. The intensity of the diphasic change was related to the [H2CO3] and so to the efficiency of the stimulus for development. 2. MES and HEPES buffers induced a rapid rise in pHi of infective stages in step with pHo. Ortho-and pyrophosphate induced greater changes in pHi. Such buffers did not initiate development. 3. These and other results suggest that the stimulus for development, H2CO3, induced an energy-dependent Ca2+/H+ exchange mediated by mitochondria of the infective stage, which initiated development of the parasitic stage.

宿主信号H2CO3可诱导捻转血矛线虫（Haemonchus contortus）第一个寄生阶段的钙离子依赖性发育（诱导时间小于25分钟），在用5,5 - 二甲基 - 2,4 - 恶唑烷二酮测量感染阶段的细胞内pH值（pHi）时，H2CO3会在30分钟内引起pHi的双相变化。双相变化的强度与[H2CO3]相关，因此也与发育刺激的效率相关。2. MES和HEPES缓冲液会使感染阶段的pHi随细胞外pH值（pHo）同步快速升高。正磷酸盐和焦磷酸盐会引起pHi的更大变化。这些缓冲液不会引发发育。3. 这些及其他结果表明，发育刺激物H2CO3会诱导感染阶段线粒体介导的能量依赖性Ca2+/H+交换，从而启动寄生阶段的发育。