Tembo Rabecca, Kaile Trevor, Kafita Doris, Chisanga Chrispin, Kalonda Annie, Zulu Ephraim, Samutela Mulemba, Polepole Pascal, Kwenda Geoffrey
Department of Pathology and Microbiology, School of Medicine, University of Zambia, Lusaka, Zambia.
Department of Biomedical Sciences, School of Health Sciences, University of Zambia, Lusaka, Zambia.
Pan Afr Med J. 2017 Jun 22;27:137. doi: 10.11604/pamj.2017.27.137.11845. eCollection 2017.
Human herpes virus-8, a γ2-herpes virus, is the aetiological agent of Kaposi sarcoma. Recently, Kaposi's sarcoma cases have increased in Zambia. However, the diagnosis of this disease is based on morphological appearance of affected tissues using histological techniques, and the association with its causative agent, Human Herpes virus 8 is not sought. This means poor prognosis for affected patients since the causative agent is not targeted during diagnosis and KS lesions may be mistaken for other reactive and neoplastic vascular proliferations when only histological techniques are used. Therefore, this study was aimed at providing evidence of Human Herpes virus 8 infection in Kaposi's sarcoma tissues at the University Teaching Hospital in Lusaka, Zambia.
One hundred and twenty suspected Kaposi's sarcoma archival formalin-fixed paraffin-wax embedded tissues stored from January 2013 to December 2014 in the Histopathology Laboratory at the University Teaching Hospital, Lusaka, Zambia were analysed using histology and Polymerase Chain Reaction targeting the ORF26 gene of Human Herpes virus 8.
The predominant histological type of Kaposi's sarcoma detected was the Nodular type (60.7%) followed by the plaque type (22.6%) and patch type (16.7%). The nodular lesion was identified mostly in males (40.5%, 34/84) than females (20.2%, 17/84) (p=0.041). Human Herpes virus 8 DNA was detected in 53.6% (45/84) and mostly in the nodular KS lesions (60%, 27/84) (p=0.035).
The findings in this study show that the Human Herpes virus-8 is detectable in Kaposi's sarcoma tissues, and, as previously reported in other settings, is closely associated with Kaposi's sarcoma. The study has provided important baseline data for use in the diagnosis of this disease and the identification of the virus in the tissues will aid in targeted therapy.
人类疱疹病毒8型是一种γ2疱疹病毒，是卡波西肉瘤的病原体。最近，赞比亚的卡波西肉瘤病例有所增加。然而，该疾病的诊断是基于使用组织学技术观察受影响组织的形态外观，并未探寻其与病原体人类疱疹病毒8型的关联。这意味着受影响患者的预后较差，因为在诊断过程中未针对病原体，并且仅使用组织学技术时，卡波西肉瘤病变可能会被误诊为其他反应性和肿瘤性血管增生。因此，本研究旨在为赞比亚卢萨卡大学教学医院卡波西肉瘤组织中的人类疱疹病毒8型感染提供证据。
对2013年1月至2014年12月储存在赞比亚卢萨卡大学教学医院组织病理学实验室的120份疑似卡波西肉瘤存档福尔马林固定石蜡包埋组织进行组织学分析，并采用聚合酶链反应靶向人类疱疹病毒8型的ORF26基因。
检测到的卡波西肉瘤的主要组织学类型为结节型（60.7%），其次是斑块型（22.6%）和斑片型（16.7%）。结节性病变在男性中更为常见（40.5%，34/84），高于女性（20.2%，17/84）（p=0.041）。在53.6%（45/84）的样本中检测到人类疱疹病毒8型DNA，且大多存在于结节性卡波西肉瘤病变中（60%，27/84）（p=0.035）。
本研究结果表明，人类疱疹病毒8型在卡波西肉瘤组织中可检测到，并且如先前在其他环境中所报道的，与卡波西肉瘤密切相关。该研究为该疾病的诊断提供了重要的基线数据，并且在组织中鉴定出该病毒将有助于靶向治疗。
