通过电子探针X射线微量分析对射精的家禽、公牛、大鼠、狗和公猪精子的元素组成进行比较研究。

Comparative studies of elemental composition on ejaculated fowl, bull, rat, dog and boar spermatozoa by electron probe X-ray microanalysis.

Ashizawa K, Ozawa Y, Okauchi K

Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University, Japan.

Comp Biochem Physiol A Comp Physiol. 1987;88(2):269-72. doi: 10.1016/0300-9629(87)90482-8.

Abstract

Comparative analyses of the concentrations of bulk and trace elements on the head, midpiece and tail regions of the ejaculated fowl, bull, rat, dog and boar spermatozoa were performed using X-ray microanalysis with scanning electron microscopy. 2. Although the pattern of distribution of elements on the surface of the three different subcellular regions was, in general, similar among all the species, there were substantial shifts in absolute concentrations. 3. Concentration of magnesium on the dog spermatozoa was significantly higher (about 2 times) than those of the other species. 4. Zinc, copper, iron and manganese concentrations were higher on fowl spermatozoa compared with those of the other species studied. 5. The Na-to-K ratios on the midpiece ranged from 1.46 (rat) to 2.26 (dog).

摘要

使用扫描电子显微镜的X射线微量分析技术，对射精的家禽、公牛、大鼠、狗和公猪精子头部、中段和尾部区域的大量元素和微量元素浓度进行了比较分析。2. 尽管在所有物种中，三种不同亚细胞区域表面的元素分布模式总体相似，但绝对浓度存在显著变化。3. 狗精子上镁的浓度明显高于其他物种（约为其他物种的2倍）。4. 与所研究的其他物种相比，家禽精子上锌、铜、铁和锰的浓度更高。5. 中段的钠钾比范围为1.46（大鼠）至2.26（狗）。