Wang Quanxiu, Chen Pingli, Wang Honglin, Chao Shuangshuang, Guo Wenru, Zhang Yuxue, Miao Chenglin, Yuan Hongyu, Peng Bo
College of Life Sciences, Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, 464000 China.
Guangdong Key Laboratory of New Technology in Rice Breeding, The Rice Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 China.
Mol Breed. 2023 May 5;43(5):38. doi: 10.1007/s11032-023-01387-z. eCollection 2023 May.
The photosystem II (PSII) outer antenna LHCB3 protein plays critical roles in distributing the excitation energy and modulating the rate of state transition for photosynthesis. Here, knockdown mutants were produced using the RNAi system. Phenotypic analyses showed that knockdown led to pale green leaves and lower chlorophyll contents at both tillering and heading stages. In addition, mutant lines exhibited decreased non-photochemical quenching (NPQ) capacity and net photosynthetic rate (Pn) by downregulating the expression of PSII-related genes. Moreover, RNA-seq experiments were performed at both tillering and heading stages. The differentially expressed genes (DEGs) mainly involved in chlorophyll binding response to abscisic acid, photosystem II, response to chitin, and DNA-binding transcription factor. Besides, our transcriptomic and physiological data indicated that was essential for binding chlorophyll, but not for the metabolism of chlorophyll in rice. RNAi knockdown plants affected the expression of PS II-related genes, but not PS I-related genes. Overall, these results suggest that also plays vital roles in regulating photosynthesis and antenna proteins in rice as well as responses to environment stresses.
The online version contains supplementary material available at 10.1007/s11032-023-01387-z.
光系统II（PSII）外周天线LHCB3蛋白在光合作用中激发能的分配和状态转换速率的调节方面发挥着关键作用。在此，利用RNA干扰系统构建了基因敲除突变体。表型分析表明，基因敲除导致分蘖期和抽穗期叶片呈淡绿色且叶绿素含量降低。此外，突变株系通过下调PSII相关基因的表达，表现出非光化学猝灭（NPQ）能力和净光合速率（Pn）下降。此外，在分蘖期和抽穗期均进行了RNA测序实验。差异表达基因（DEG）主要涉及对脱落酸的叶绿素结合反应、光系统II、对几丁质的反应以及DNA结合转录因子。此外，我们的转录组和生理数据表明，该基因对水稻叶绿素结合至关重要，但对叶绿素代谢并非必需。RNA干扰敲除植株影响PS II相关基因的表达，但不影响PS I相关基因的表达。总体而言，这些结果表明该基因在调节水稻光合作用和天线蛋白以及对环境胁迫的响应中也起着至关重要的作用。
在线版本包含可在10.1007/s11032-023-01387-z获取的补充材料。
