Yang Chong, Xu Ling, Zhang Na, Islam Faisal, Song Wenjian, Hu Luyang, Liu Dan, Xie Xiaonan, Zhou Weijun
Zhejiang Key Laboratory of Crop Germplasm, Institute of Crop Science, Zhejiang University, Hangzhou, P. R. China.
Zhejiang Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, P. R. China.
Proteomics. 2017 Jul;17(13-14). doi: 10.1002/pmic.201700009.
Orobanche cumana is an obligate root parasite causing severe damage to many economically important crops, including sunflowers worldwide. For efficient control measures, it is necessary to understand the resistant mechanism during interaction at molecular level. The present study emphasizes on comparative proteomics to investigate the mechanistic basis of compatible and incompatible interaction of O. cumana with resistant (JY207) and susceptible (TK0409) sunflowers. More than 3500 proteins were identified from two cultivars by iTRAQ analysis. Identified proteins associated with general functions, posttranslational modification, energy production and conversion, carbohydrate transport and metabolism, and signal transduction mechanisms were the most represented category of induced proteins in both cultivars. The resistant interaction was characterized by alteration of defense-related proteins involved in recognition of parasites, accumulation of pathogenesis-related proteins, biosynthesis of lignin, and detoxification of toxic metabolites in JY207 after inoculation. The susceptible interaction was characterized by decreased abundance of proteins involved in biosynthesis and signaling of plant growth regulators including auxin, gibberellin, brassinosteroid, and ethylene in TK0409 after inoculation. The present study provides comprehensive details of proteins and differential modulation of pathways regulated under compatible and incompatible interaction, allowing the identification of important molecular components for development of sustainable resistance against this parasite.
列当是一种专性根寄生植物，对包括全球范围内的向日葵在内的许多经济作物造成严重损害。为了采取有效的防治措施，有必要在分子水平上了解相互作用过程中的抗性机制。本研究着重于比较蛋白质组学，以研究列当与抗性（JY207）和感病（TK0409）向日葵之间亲和与非亲和相互作用的机制基础。通过iTRAQ分析从两个品种中鉴定出3500多种蛋白质。在两个品种中，鉴定出的与一般功能、翻译后修饰、能量产生和转换、碳水化合物运输和代谢以及信号转导机制相关的蛋白质是诱导蛋白中最主要的类别。抗性相互作用的特征是，接种后JY207中参与识别寄生虫的防御相关蛋白发生改变、病程相关蛋白积累、木质素生物合成以及有毒代谢物解毒。感病相互作用的特征是，接种后TK0409中参与植物生长调节剂（包括生长素、赤霉素、油菜素内酯和乙烯）生物合成和信号传导的蛋白质丰度降低。本研究提供了亲和与非亲和相互作用下蛋白质的全面细节以及途径的差异调节，有助于鉴定出对该寄生虫产生可持续抗性的重要分子成分。
