The Herbal Medicinal Products Research and Development Center (cooperation between Rangsit University, Harbin Institute of Technology, and Heilongjiang University of Chinese Medicine), Faculty of Pharmacy, Rangsit University, Muang, Pathum Thani, Thailand.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Rangsit University, Muang, Pathum Thani, Thailand.
J Food Drug Anal. 2016 Jul;24(3):493-499. doi: 10.1016/j.jfda.2016.01.007. Epub 2016 Feb 23.
The aim of this work was to validate the simple and rapid isocratic reversed phase-high performance liquid chromatography using a C-18 column for the determination of curcuminoid contents, dissolution profile, and volatile oil content of turmeric capsules produced at three secondary government hospitals. The validated reversed phase-high performance liquid chromatography method for three curcuminoids (bisdemethoxycurcumin, demethoxycurcumin, and curcumin) had a good linearity (R > 0.9990), accuracy (% recovery was 99.96-101.14%, 97.42-102.23%, and 98.01-99.12% for bisdemethoxycurcumin, demethoxycurcumin, and curcumin, respectively), precision (% relative standard deviation < 2% and < 5% for intraday and interday precision, respectively), including limit of detection, limit of quantitation, and system suitability. We found that turmeric capsules had a higher content of curcumin than bisdemethoxycurcumin and demethoxycurcumin. The total curcuminoid contents of all lots ranged from 12.02%w/w to 14.36%w/w. Dissolution profiles of curcuminoids were fitted with Higuchi model. Moreover, volatile oil content, determined using the hydrodistillation method, ranged from 7.00%v/w to 8.00%v/w. In conclusion, all nine lots of turmeric capsules from three secondary government hospitals met the standard criteria of the Thai Herbal Pharmacopoeia in the topic of curcuminoid contents, dissolution, and volatile oil content.
本工作旨在验证简单快速的反相高效液相色谱法，使用 C-18 柱测定在三所政府二级医院生产的姜黄胶囊中的姜黄素含量、溶出度和挥发油含量。经验证的反相高效液相色谱法可同时测定三种姜黄素（双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素），具有良好的线性（R>0.9990）、准确度（%回收率分别为 99.96-101.14%、97.42-102.23%和 98.01-99.12%）、精密度（日内和日间精密度的%相对标准偏差均<2%和<5%），包括检测限、定量限和系统适用性。我们发现姜黄胶囊中的姜黄素含量高于双去甲氧基姜黄素和去甲氧基姜黄素。所有批次的总姜黄素含量范围为 12.02%w/w 至 14.36%w/w。姜黄素的溶出度曲线符合 Higuchi 模型。此外，使用水蒸馏法测定的挥发油含量范围为 7.00%v/w 至 8.00%v/w。总之，来自三所政府二级医院的九批姜黄胶囊在姜黄素含量、溶出度和挥发油含量方面均符合泰国草药药典的标准。
