Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si 28116, Korea.
Department of Biodiversity and Bioresources, Satvik Nepal, Dadakonak-27, Pokhara 33700, Nepal.
Molecules. 2019 May 10;24(9):1805. doi: 10.3390/molecules24091805.
A fast and reliable ultra-performance liquid chromatography-diode array detection method was developed and validated for the quantitative assessment of turmeric extracts from different geographical locations. Acclaim RSLC PolarAdvantage II column (2.2 μm, 2.1 × 100 mm) was used to analyze individual curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) from turmeric samples. The detection was done on ultraviolet absorbance at 425 nm and the column temperature was maintained at 45 °C. A mobile phase consisting of acetonitrile and water was found to be suitable for separation, at a flow rate of 1 mL/min with linear gradient elution. Linearity, specificity, precision, recovery and robustness were measured to validate the method and instrument. Under the described conditions, curcuminoids were collected within one minute. The calibration curve of each curcuminoid showed good linearity (correlation coefficient > 0.999). The relative standard deviations (RSD) of intra-day, inter-day precision and repeatability were less than 0.73%, 2.47% and 2.47%, respectively. In the recovery test, the accuracy ranged from 98.54%-103.91% with RSD values of less than 2.79%. The developed method was used for quantification of individual curcuminoids of turmeric samples. Analysis of turmeric samples from Nepal and South Korea revealed that curcuminoid content was related to geographical location. Turmeric cultivated in warmer climates were found to have higher curcumionoid content than turmeric samples from cooler climates, the southern part of Nepal was found to have two times higher content of curcuminoids than turmeric from the north.
建立并验证了一种快速可靠的超高效液相色谱-二极管阵列检测法，用于定量评估来自不同地理位置的姜黄提取物。采用 Acclaim RSLC PolarAdvantage II 柱（2.2μm，2.1×100mm）分析姜黄样品中的单个姜黄素（姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素）。在 425nm 紫外吸收处进行检测，柱温保持在 45°C。结果表明，乙腈和水组成的流动相适合分离，流速为 1mL/min，采用线性梯度洗脱。对方法和仪器进行了线性、专属性、精密度、回收率和耐用性的测定。在所述条件下，姜黄素类化合物在一分钟内得到收集。每个姜黄素类化合物的校准曲线均表现出良好的线性（相关系数>0.999）。日内、日间精密度和重复性的相对标准偏差（RSD）分别小于 0.73%、2.47%和 2.47%。在回收率试验中，准确度在 98.54%-103.91%之间，RSD 值小于 2.79%。所建立的方法用于姜黄样品中单个姜黄素类化合物的定量分析。对来自尼泊尔和韩国的姜黄样品的分析表明，姜黄素类化合物的含量与地理位置有关。在较温暖气候下种植的姜黄被发现比在较凉爽气候下种植的姜黄具有更高的姜黄素类化合物含量，尼泊尔南部的姜黄素类化合物含量是北部的两倍。
