Navasconi Taisa Rocha, Dos Reis Vanessa Nesi, Freitas Camila Fabiano, Pereira Paulo Cesar de Souza, Caetano Wilker, Hioka Noboru, Lonardoni Maria Valdrinez Campana, Aristides Sandra Mara Alessi, Silveira Thaís Gomes Verzignassi
Graduate Program in Health Sciences, Universidade Estadual de Maringá, Maringá, Brazil.
Graduate Program in Chemistry, Universidade Estadual de Maringá, Maringá, Brazil.
J Lasers Med Sci. 2017 Winter;8(1):46-50. doi: 10.15171/jlms.2017.09. Epub 2017 Jan 8.
The treatment of cutaneous leishmaniasis (CL) is based primarily on the use of pentavalent antimonials, which may lead to many side effects limiting their use. Photodynamic therapy (PDT) is an alternative for the treatment of CL, and some xanthene dyes have the potential for use in PDT. The xanthenes rose bengal B (RB) and its derivatives rose bengal methyl ester (RBMET), and butyl ester (RBBUT) were analyzed for leishmanicidal activity against promastigotes and intracellular amastigotes of Leishmania amazonensis. Cytotoxicity was assessed in J774.A1 macrophages. RB derivates RBMET (IC 9.83 μM), and RBBUT (IC 45.08 μM) showed leishmanicidal activity, however, were toxic to J774.A1 macrophages, resulting in low selectivity index. The RBMET and RBBUT showed to be effective against the L. amazonensis and the low selectivity index presented may not be a limitation for their use in PDT to CL treatment.
皮肤利什曼病（CL）的治疗主要基于使用五价锑化合物，但这可能会导致许多副作用，限制了它们的使用。光动力疗法（PDT）是治疗CL的一种替代方法，一些呫吨染料有用于PDT的潜力。分析了呫吨类的孟加拉玫瑰红B（RB）及其衍生物孟加拉玫瑰红甲酯（RBMET）和丁酯（RBBUT）对亚马逊利什曼原虫前鞭毛体和细胞内无鞭毛体的杀利什曼活性。在J774.A1巨噬细胞中评估细胞毒性。RB衍生物RBMET（IC 9.83 μM）和RBBUT（IC 45.08 μM）显示出杀利什曼活性，然而，它们对J774.A1巨噬细胞有毒性，导致选择性指数较低。RBMET和RBBUT对亚马逊利什曼原虫有效，并且所呈现的低选择性指数可能并不限制它们在PDT治疗CL中的使用。
