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无症状 HBsAg 携带者和慢性乙型肝炎患者细胞 microRNA 的表达谱分析。

Expression Profiling of Cellular MicroRNA in Asymptomatic HBsAg Carriers and Chronic Hepatitis B Patients.

机构信息

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.

出版信息

Biomed Res Int. 2017;2017:6484835. doi: 10.1155/2017/6484835. Epub 2017 Aug 23.

DOI:10.1155/2017/6484835
PMID:28913356
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5587942/
Abstract

BACKGROUND

MicroRNAs (miRNAs) may serve as potential molecular markers to predict liver injury resulting from chronic hepatitis B (CHB). In the present study, we want to study the expression profile and clinical significance of miRNAs at different stages of CHB virus infection.

METHODS

Using miRNA microarray, we investigated the global expression profiles of cellular miRNA in asymptomatic hepatitis B antigen carriers (ASCs) and CHB patients, compared with healthy controls (HCs).

RESULTS

We identified 79 and 203 differentially expressed miRNAs in the peripheral blood mononuclear cells of ASCs and CHB patients compared to HCs, respectively. Some of these miRNAs were common to ASCs and CHB patients, but another set of miRNAs that showed differential expression between ASCs and CHB patients was also identified. Gene ontology and pathway enrichment analysis showed that the target genes of the identified miRNAs played a role in important biological functions, such as learning or memory, cell-cell adherens junction, ion channel inhibitor activity, TGF-beta signaling pathway, and p53 signaling pathway.

CONCLUSION

We identified some significant differentially expressed miRNA in different phases of HBV infection, which might serve as biomarkers or therapeutic targets in the future.

摘要

背景

MicroRNAs (miRNAs) 可能作为预测慢性乙型肝炎 (CHB) 导致肝损伤的潜在分子标志物。在本研究中，我们希望研究乙型肝炎病毒感染不同阶段细胞 miRNA 的表达谱和临床意义。

方法

使用 miRNA 微阵列，我们研究了无症状乙型肝炎抗原携带者 (ASCs) 和 CHB 患者与健康对照 (HCs) 相比外周血单个核细胞中细胞 miRNA 的全局表达谱。

结果

我们分别在 ASC 和 CHB 患者的外周血单个核细胞中鉴定出 79 个和 203 个差异表达的 miRNA。这些 miRNA 中的一些在 ASCs 和 CHB 患者中是共同的，但也鉴定出另一组在 ASCs 和 CHB 患者之间差异表达的 miRNA。基因本体和通路富集分析表明，鉴定出的 miRNA 的靶基因在学习或记忆、细胞-细胞黏附连接、离子通道抑制剂活性、TGF-β 信号通路和 p53 信号通路等重要生物学功能中发挥作用。

结论

我们在 HBV 感染的不同阶段鉴定出一些显著差异表达的 miRNA，它们可能成为未来的生物标志物或治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/6edd39c61c54/BMRI2017-6484835.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/1df642b50ae0/BMRI2017-6484835.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/4a3602e9131e/BMRI2017-6484835.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/7365360aa228/BMRI2017-6484835.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/2e97dbd93e92/BMRI2017-6484835.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/e22bf62ab992/BMRI2017-6484835.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/c23bdd69d934/BMRI2017-6484835.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/6edd39c61c54/BMRI2017-6484835.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/1df642b50ae0/BMRI2017-6484835.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/4a3602e9131e/BMRI2017-6484835.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/7365360aa228/BMRI2017-6484835.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/2e97dbd93e92/BMRI2017-6484835.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/e22bf62ab992/BMRI2017-6484835.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/c23bdd69d934/BMRI2017-6484835.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ede1/5587942/6edd39c61c54/BMRI2017-6484835.007.jpg

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