一种控制等温核酸扩增中残留污染的新方法。
A novel method to control carryover contamination in isothermal nucleic acid amplification.
作者信息
Ma Cuiping, Wang Fuxin, Wang Xiudan, Han Lingzhi, Jing Hao, Zhang Heng, Shi Chao
机构信息
College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, P. R. China.
出版信息
Chem Commun (Camb). 2017 Sep 26;53(77):10696-10699. doi: 10.1039/c7cc06469a.
PMID:28913529
Abstract
We developed a novel method to control carryover contamination in loop-mediated isothermal amplification (LAMP) by primer engineering to carry recognition sites for a restriction endonuclease, providing a robust ability to eliminate carryover contaminants.
摘要
我们开发了一种新方法，通过引物工程在环介导等温扩增（LAMP）中控制残留污染，使引物携带限制性内切酶的识别位点，从而具备强大的消除残留污染物的能力。
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