除草剂稀禾啶和高效盖草能对植物乙酰辅酶A羧化酶的抑制作用。

Inhibition of plant acetyl-coenzyme A carboxylase by the herbicides sethoxydim and haloxyfop.

作者信息

Burton J D, Gronwald J W, Somers D A, Connelly J A, Gengenbach B G, Wyse D L

机构信息

Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul 55108.

出版信息

Biochem Biophys Res Commun. 1987 Nov 13;148(3):1039-44. doi: 10.1016/s0006-291x(87)80236-x.

DOI:10.1016/s0006-291x(87)80236-x

PMID:2891354

Abstract

Incorporation of [14C]acetate or [14C]pyruvate into fatty acids in isolated corn seedling chloroplasts was inhibited 90% or greater by 10 microM sethoxydim or 1 microM haloxyfop. At these concentrations, neither sethoxydim nor haloxyfop inhibited [14C]acetate incorporation into fatty acids in isolated pea chloroplasts. Sethoxydim (10 microM) and haloxyfop (1 microM) did not inhibit incorporation of [14C]malonyl-CoA into fatty acids in cell free extracts from corn tissue cultures. Acetyl coenzyme A carboxylase (EC 6.4.1.2) from corn seedling chloroplasts was inhibited by both sethoxydim and haloxyfop, with I50 values of 2.9 and 0.5 microM, respectively. This enzyme in pea was not inhibited by 10 microM sethoxydim or 1 microM haloxyfop.

摘要

在离体玉米幼苗叶绿体中，10微摩尔的稀禾啶或1微摩尔的氯氟吡氧乙酸能抑制[14C]乙酸盐或[14C]丙酮酸掺入脂肪酸，抑制率达90%或更高。在这些浓度下，稀禾啶和氯氟吡氧乙酸均不抑制[14C]乙酸盐掺入离体豌豆叶绿体中的脂肪酸。10微摩尔的稀禾啶和1微摩尔的氯氟吡氧乙酸不抑制[14C]丙二酰辅酶A掺入玉米组织培养物的无细胞提取物中的脂肪酸。玉米幼苗叶绿体中的乙酰辅酶A羧化酶（EC 6.4.1.2）受到稀禾啶和氯氟吡氧乙酸的抑制，其半数抑制浓度（I50）值分别为2.9微摩尔和0.5微摩尔。豌豆中的这种酶不受10微摩尔稀禾啶或1微摩尔氯氟吡氧乙酸的抑制。