Gornicki P, Haselkorn R
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
Plant Mol Biol. 1993 Jun;22(3):547-52. doi: 10.1007/BF00015984.
The acetyl-CoA carboxylase present in both wheat germ and total wheat leaf protein contains ca. 220 kDa subunits. It is the major biotin-dependent carboxylase present in wheat chloroplasts. Active acetyl-CoA carboxylase purified from wheat germ is a homodimer with an apparent molecular mass of ca. 500 kDa. The enzyme from wheat germ or from wheat chloroplasts is sensitive to the herbicide haloxyfop at micromolar levels. The incorporation of 14C-acetate into fatty acids in freshly cut wheat seedling leaves provides a convenient in vivo assay for both acetyl-CoA carboxylase and haloxyfop.
小麦胚芽和全叶蛋白中的乙酰辅酶A羧化酶含有约220 kDa的亚基。它是小麦叶绿体中主要的生物素依赖性羧化酶。从小麦胚芽中纯化得到的活性乙酰辅酶A羧化酶是一种同型二聚体，表观分子量约为500 kDa。从小麦胚芽或小麦叶绿体中提取的该酶对微摩尔浓度的除草剂氯氟吡氧乙酸敏感。将14C-乙酸掺入新切小麦幼苗叶片的脂肪酸中，为乙酰辅酶A羧化酶和氯氟吡氧乙酸提供了一种便捷的体内检测方法。
