Mishra Ankita, Arora Naveen
Allergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology, Room 509, Delhi University Campus, Mall Road, Delhi, 110007, India.
Methods Mol Biol. 2017;1679:97-111. doi: 10.1007/978-1-4939-7337-8_6.
Agriculture biotechnology is a promising tool for developing varieties with enhanced quality and quantity. Transgenic proteins expressed by genetically modified (GM) food crops improve crop characteristics like nutritional value, taste, and texture, and endow plants with resistance against fungus, pests, and insects. Despite such potential benefits, there are concerns regarding possible adverse effects of GM crops on human health, animals and the environment. Among the proposed guidelines for GM food safety testing-the weight-of-evidence approach proposed by the Codex Alimentarius Commission (ALINORM 03/34A) is the most recent. Till date, several transgenic wheat lines have been developed and research is underway for further improvement. However, GM wheat is not being grown or consumed in any part of the world. In the present study, in silico tools were employed for safety testing of eight transgenes used for the development of transgenic wheat lines. Among the genes studied, none of them shared sequence homology with the reported allergens and may be safe for use in genetic engineering. In conclusion, gene selection for developing transgenic wheat lines should be done with utmost care to ensure its safety for feed and fodder.
农业生物技术是培育品质和产量均得到提升的品种的一项很有前景的工具。转基因粮食作物表达的转基因蛋白能改善作物特性，如营养价值、口感和质地，并使植物具备抗真菌、抗害虫和抗昆虫的能力。尽管有这些潜在益处，但人们仍担心转基因作物可能对人类健康、动物和环境产生不利影响。在拟议的转基因食品安全检测准则中，食品法典委员会提出的证据权重法（ALINORM 03/34A）是最新的方法。迄今为止，已经培育出了多个转基因小麦品系，并且正在进行进一步改良的研究。然而，世界上任何地方都没有种植或食用转基因小麦。在本研究中，利用计算机工具对用于培育转基因小麦品系的8个转基因进行了安全性检测。在所研究的基因中，没有一个与已报道的过敏原具有序列同源性，可能可安全用于基因工程。总之，培育转基因小麦品系时的基因选择应格外谨慎，以确保其用作饲料的安全性。
