Burridge Amanda J, Winfield Mark O, Allen Alexandra M, Wilkinson Paul A, Barker Gary L A, Coghill Jane, Waterfall Christy, Edwards Keith J
School of Biological Sciences, University of Bristol, Bristol Life Sciences Building, 24 Tyndall Ave, Bristol, BS8 1TH, UK.
Methods Mol Biol. 2017;1679:293-306. doi: 10.1007/978-1-4939-7337-8_19.
A lack of genetic diversity between wheat breeding lines has been recognized as a significant block to future yield increases. Wheat breeding and prebreeding strategies are increasingly using material from wheat ancestors or wild relatives to reintroduce diversity. Where molecular markers are polymorphic between the host and introgressed material, they may be used to track the size and location of the introgressed material through generations of backcrossing. To generate markers for this purpose, sequence capture targeted resequencing was carried out for a range of wheat varieties, wheat relatives, and wheat progenitors. From these sequences, putative SNPs were identified and used to generate the Axiom® Wheat HD array. A selection of varieties representing a selection of elite wheat breeding material, progenitor species, and wild relatives were used to validate the array. The procedures used are described here in detail.
小麦育种系之间缺乏遗传多样性已被认为是未来产量增长的重大障碍。小麦育种和预育种策略越来越多地利用小麦祖先或野生近缘种的材料来重新引入多样性。当分子标记在宿主和渗入材料之间具有多态性时，它们可用于通过回交世代追踪渗入材料的大小和位置。为了生成用于此目的的标记，对一系列小麦品种、小麦近缘种和小麦祖先进行了序列捕获靶向重测序。从这些序列中，鉴定出推定的单核苷酸多态性（SNP）并用于生成Axiom®小麦高清芯片。选择了代表一系列优良小麦育种材料、祖先物种和野生近缘种的品种来验证该芯片。这里详细描述了所使用的程序。
