a Department of Ophthalmology , College of Medicine, King Saud University , Riyadh , Saudi Arabia.
b Dr. Nasser Al-Rashid Research Chair in Ophthalmology, College of Medicine, King Saud University , Riyadh , Saudi Arabia.
Ocul Immunol Inflamm. 2018;26(8):1248-1260. doi: 10.1080/09273948.2017.1343855. Epub 2017 Sep 15.
We investigated the expression of the proinflammatory and proangiogenic factor osteoprotegerin (OPG) and its ligands, receptor activator of nuclear factor-κB ligand (RANKL), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), and the receptor RANK in proliferative diabetic retinopathy (PDR).
Vitreous samples from PDR and nondiabetic control patients and epiretinal membranes from PDR patients were studied by enzyme-linked immunosorbent assay, immunohistochemistry, and Western blot analysis.
Vascular endothelial growth factor, OPG, and soluble RANK levels in vitreous samples from PDR patients were significantly higher than that in nondiabetic controls. Soluble TRAIL levels were significantly lower in PDR patients than that in nondiabetic control, whereas soluble RANKL levels did not differ significantly. RANKL, RANK, and TRAIL were expressed in vascular endothelial cells, myofibroblasts, and CD45-expressing leukocytes in PDR epiretinal membranes.
Dysregulated expression of OPG/RANKL/RANK pathway and TRAIL might be related to inflammation and angiogenesis in PDR.
我们研究了促炎和促血管生成因子骨保护素（OPG）及其配体核因子-κB 受体激活剂配体（RANKL）、肿瘤坏死因子相关凋亡诱导配体（TRAIL）和受体 RANK 在增殖性糖尿病视网膜病变（PDR）中的表达。
通过酶联免疫吸附试验、免疫组织化学和 Western blot 分析研究了来自 PDR 和非糖尿病对照患者的玻璃体样本以及来自 PDR 患者的视网膜前膜。
来自 PDR 患者的玻璃体样本中的血管内皮生长因子、OPG 和可溶性 RANK 水平明显高于非糖尿病对照组。PDR 患者的可溶性 TRAIL 水平明显低于非糖尿病对照组，而可溶性 RANKL 水平没有显著差异。RANKL、RANK 和 TRAIL 在 PDR 视网膜前膜中的血管内皮细胞、肌成纤维细胞和表达 CD45 的白细胞中表达。
OPG/RANKL/RANK 通路和 TRAIL 的失调表达可能与 PDR 中的炎症和血管生成有关。
