• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

miR-217 通过 OPG/RANKL/RANK 在破骨细胞瘤中调控自噬。

MiR-217 regulates autophagy through OPG/RANKL/RANK in giant cell tumors.

机构信息

The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010030, China.

Bao Tou Medical College, Baotou, 014040, China.

出版信息

J Orthop Surg Res. 2023 May 10;18(1):346. doi: 10.1186/s13018-023-03826-1.

DOI:10.1186/s13018-023-03826-1
PMID:37165403
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10170763/
Abstract

BACKGROUND

Increasing evidence suggests that microRNAs (miRNAs) play a crucial role in cancer development and progression. Our previous study showed remarkably lower levels of miR-217 in GCT cells and tissues, and miR-217 re-expression inhibited the occurrence and development of GCT in vitro; however, the associated mechanisms remain unknown. Thus, this study aimed to explore the mechanisms underlying the proliferation inhibitory effect of miR-217 in GCT cells.

METHODS

The proliferative potential of the GCT cells was measured with an MTT assay and BrdU straining. Changes in GCT cell migration and invasion was assessed by a transwell assay. Finally, Western blot and RT-PCR assays were employed to evaluate OPG/RANKL/RANK signaling pathway-related protein expression.

RESULTS

The excessive upregulation of miR-217 markedly suppressed GCT cell proliferation and tumorigenesis both in vitro and in vivo. miR-217 overexpression could inhibit the OPG/RANKL/RANK signaling pathway in vitro and in vivo. Furthermore, ALP activity was significantly decreased in GCT cells following miR-217 treatment. Importantly, miR-217 could inhibit autophagy-related protein expression and autophagosome/autolysosome formation in GCT cells and tissues.

CONCLUSION

These results suggest that miR-217 upregulation could inhibit the occurrence and development of GCT by blocking autophagy. These findings offer an effective therapeutic target to improve the survival rates of patients with CGT in the future.

摘要

背景

越来越多的证据表明 microRNAs（miRNAs）在癌症的发生和发展中起着至关重要的作用。我们之前的研究表明，miR-217 在 GCT 细胞和组织中的水平明显降低，miR-217 的重新表达抑制了 GCT 在体外的发生和发展；然而，相关机制尚不清楚。因此，本研究旨在探讨 miR-217 抑制 GCT 细胞增殖的机制。

方法

用 MTT 法和 BrdU 染色法测定 GCT 细胞的增殖潜力。通过 Transwell 测定法评估 GCT 细胞迁移和侵袭的变化。最后，采用 Western blot 和 RT-PCR 法评估 OPG/RANKL/RANK 信号通路相关蛋白的表达。

结果

miR-217 的过度上调显著抑制了 GCT 细胞在体外和体内的增殖和肿瘤发生。miR-217 的过表达可以抑制体外和体内的 OPG/RANKL/RANK 信号通路。此外，miR-217 处理后 GCT 细胞中的 ALP 活性显著降低。重要的是，miR-217 可以抑制 GCT 细胞和组织中自噬相关蛋白的表达和自噬体/自溶体的形成。

结论

这些结果表明，miR-217 的上调可以通过阻断自噬来抑制 GCT 的发生和发展。这些发现为未来提高 CGT 患者的生存率提供了一个有效的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/e17ca01e7e0c/13018_2023_3826_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/d94804b7ff08/13018_2023_3826_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/f84f8019b11f/13018_2023_3826_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/97977cd762ca/13018_2023_3826_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/cebb9a43451c/13018_2023_3826_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/3bde30e18822/13018_2023_3826_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/e17ca01e7e0c/13018_2023_3826_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/d94804b7ff08/13018_2023_3826_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/f84f8019b11f/13018_2023_3826_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/97977cd762ca/13018_2023_3826_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/cebb9a43451c/13018_2023_3826_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/3bde30e18822/13018_2023_3826_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cd01/10170763/e17ca01e7e0c/13018_2023_3826_Fig6_HTML.jpg

相似文献

1
MiR-217 regulates autophagy through OPG/RANKL/RANK in giant cell tumors.miR-217 通过 OPG/RANKL/RANK 在破骨细胞瘤中调控自噬。
J Orthop Surg Res. 2023 May 10;18(1):346. doi: 10.1186/s13018-023-03826-1.
2
The Role of MicroRNA-143-5p in the Differentiation of Dental Pulp Stem Cells into Odontoblasts by Targeting Runx2 via the OPG/RANKL Signaling Pathway.microRNA-143-5p 通过靶向 OPG/RANKL 信号通路调控 Runx2 对牙髓干细胞向成牙本质细胞分化的作用
J Cell Biochem. 2018 Jan;119(1):536-546. doi: 10.1002/jcb.26212. Epub 2017 Jul 24.
3
MicroRNA-145 Mediates Steroid-Induced Necrosis of the Femoral Head by Targeting the OPG/RANK/RANKL Signaling Pathway.微小RNA-145通过靶向骨保护素/核因子κB受体活化因子/核因子κB受体活化因子配体信号通路介导类固醇诱导的股骨头坏死。
PLoS One. 2016 Jul 26;11(7):e0159805. doi: 10.1371/journal.pone.0159805. eCollection 2016.
4
Osteoprotegerin inhibits osteoclast formation and bone resorbing activity in giant cell tumors of bone.骨保护素抑制骨巨细胞瘤中破骨细胞的形成和骨吸收活性。
Bone. 2001 Apr;28(4):370-7. doi: 10.1016/s8756-3282(01)00404-5.
5
S-amlodipine improves endothelial dysfunction via the RANK/RANKL/OPG system by regulating microRNA-155 in hypertension.S-氨氯地平通过调节高血压中的 microRNA-155 改善了 RANK/RANKL/OPG 系统相关的内皮功能障碍。
Biomed Pharmacother. 2019 Jun;114:108799. doi: 10.1016/j.biopha.2019.108799. Epub 2019 Apr 2.
6
microRNA-143-3p regulates odontogenic differentiation of human dental pulp stem cells through regulation of the osteoprotegerin-RANK ligand pathway by targeting RANK.microRNA-143-3p 通过靶向 RANK 调控核因子-κB 受体激活因子配体-核因子-κB 受体激活因子途径调控人牙髓干细胞的成牙分化。
Exp Physiol. 2020 May;105(5):876-885. doi: 10.1113/EP087992. Epub 2020 Apr 16.
7
MicroRNA-106b inhibits osteoclastogenesis and osteolysis by targeting RANKL in giant cell tumor of bone.微小RNA-106b通过靶向骨巨细胞瘤中的核因子κB受体活化因子配体抑制破骨细胞生成和骨溶解。
Oncotarget. 2015 Aug 7;6(22):18980-96. doi: 10.18632/oncotarget.4223.
8
Differential expression of the RANKL/RANK/OPG system is associated with bone metastasis in human non-small cell lung cancer.RANKL/RANK/OPG 系统的差异表达与人类非小细胞肺癌骨转移相关。
PLoS One. 2013;8(3):e58361. doi: 10.1371/journal.pone.0058361. Epub 2013 Mar 13.
9
Comparison of the anti-tumor effects of denosumab and zoledronic acid on the neoplastic stromal cells of giant cell tumor of bone.比较地舒单抗和唑来膦酸对骨巨细胞瘤肿瘤性基质细胞的抗肿瘤作用。
Connect Tissue Res. 2013;54(6):439-49. doi: 10.3109/03008207.2013.848202.
10
Endothelin-1 Activates the Notch Signaling Pathway and Promotes Tumorigenesis in Giant Cell Tumor of the Spine.内皮素-1 激活 Notch 信号通路并促进脊柱巨细胞瘤的肿瘤发生。
Spine (Phila Pa 1976). 2019 Sep 1;44(17):E1000-E1009. doi: 10.1097/BRS.0000000000003044.

引用本文的文献

1
[miRNA Is Involved in the Pathogenesis of Multiple Diseases by Targeting Osteoprotegerin].[微小RNA通过靶向骨保护素参与多种疾病的发病机制]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2024 May 20;55(3):777-782. doi: 10.12182/20240560607.

本文引用的文献

1
Small interfering RNAs in tendon homeostasis.小干扰 RNA 在肌腱稳态中的作用。
Br Med Bull. 2021 Jun 10;138(1):58-67. doi: 10.1093/bmb/ldaa040.
2
Therapeutic potential of microRNA in tendon injuries.microRNA 在肌腱损伤中的治疗潜力。
Br Med Bull. 2020 May 15;133(1):79-94. doi: 10.1093/bmb/ldaa002.
3
AMPK activation induced by promethazine increases NOXA expression and Beclin-1 phosphorylation and drives autophagy-associated apoptosis in chronic myeloid leukemia.异丙嗪诱导的 AMPK 激活增加了 NOXA 的表达和 Beclin-1 的磷酸化，并驱动慢性髓性白血病中的自噬相关凋亡。
Chem Biol Interact. 2020 Jan 5;315:108888. doi: 10.1016/j.cbi.2019.108888. Epub 2019 Nov 2.
4
Mitochondrial permeability with autophagy in lifespan shortening: a novel therapeutic target for aging.线粒体通透性与自噬在寿命缩短中的作用：一种衰老的新型治疗靶点。
Acta Biochim Biophys Sin (Shanghai). 2019 Nov 25;51(11). doi: 10.1093/abbs/gmz113. Epub 2019 Nov 4.
5
Survival and prognosis in malignant giant cell tumor of bone: A population-based analysis from 1984 to 2013.骨恶性巨细胞瘤的生存与预后：一项基于人群的1984年至2013年分析。
J Bone Oncol. 2019 Sep 11;19:100260. doi: 10.1016/j.jbo.2019.100260. eCollection 2019 Dec.
6
Denosumab Therapy Obscures Histological Features of Giant Cell Tumor of Bone.地舒单抗治疗掩盖了骨巨细胞瘤的组织学特征。
J Neuropathol Exp Neurol. 2019 Dec 1;78(12):1171-1173. doi: 10.1093/jnen/nlz100.
7
Rutin protects human periodontal ligament stem cells from TNF-α induced damage to osteogenic differentiation through suppressing mTOR signaling pathway in inflammatory environment.芦丁通过抑制炎症环境中 mTOR 信号通路保护人牙周膜干细胞免受 TNF-α诱导的成骨分化损伤。
Arch Oral Biol. 2020 Jan;109:104584. doi: 10.1016/j.archoralbio.2019.104584. Epub 2019 Oct 11.
8
MiR-337-3p suppresses proliferation of epithelial ovarian cancer by targeting PIK3CA and PIK3CB.miR-337-3p 通过靶向 PI3KCA 和 PI3KCB 抑制上皮性卵巢癌细胞的增殖。
Cancer Lett. 2020 Jan 28;469:54-67. doi: 10.1016/j.canlet.2019.10.021. Epub 2019 Oct 17.
9
Surgical resection and reconstructive techniques using autologous femoral head bone-grafting in treating partial acetabular defects arising from primary pelvic malignant tumors.采用自体股骨头骨移植的手术切除和重建技术治疗原发性骨盆恶性肿瘤引起的部分髋臼缺损。
BMC Cancer. 2019 Oct 18;19(1):969. doi: 10.1186/s12885-019-6196-x.
10
Giant cell tumor of bone: Unusual features of a rare tumor.骨巨细胞瘤：一种罕见肿瘤的不寻常特征。
Rare Tumors. 2019 Sep 25;11:2036361319878894. doi: 10.1177/2036361319878894. eCollection 2019.