Environment and Genes Related to Diseases Key Laboratory of Education Ministry, School of Public Health, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China.
State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.
Sci Rep. 2017 Sep 15;7(1):11698. doi: 10.1038/s41598-017-12043-w.
The potential applications of low temperature plasma (LTP) in wound healing have aroused the concern of many researchers. In this study, an argon atmospheric pressure plasma jet was applied to generate LTP for treatment of murine fibroblast cell (L929) cultured in vitro to investigate the effect of NF-κB pathway on fibroblast proliferation. The results showed that, compared with the control, L929 cells treated with plasma for less than 20 s had significant increases of proliferation; the productions of intracellular ROS, O and NO increased with prolongation of LTP treatment time; NF-κB pathway was activated by LTP in a proper dose range, and the expression of cyclinD1 in LTP-treated cells increased with the same trend as cell proliferation. After RNA interference to block p65 expression, with the same treatment time, RNAi-treated cells proliferated more slowly and expressed less cyclinD1 than normal cells. Furthermore, pretreatment with N-acetyl-L-cysteine (NAC) markedly prevented the plasma-induced changes in cells. In conclusion, the proliferation of L929 cells induced by LTP was closely related to NF-κB signaling pathway, which might be activated by appropriate level of intracellular ROS. These novel findings can provide some theoretical reference of LTP inducing cell proliferation and promoting wound healing.
低温等离子体（LTP）在伤口愈合中的潜在应用引起了许多研究人员的关注。在这项研究中，我们应用氩气大气压等离子体射流产生 LTP 来处理体外培养的小鼠成纤维细胞（L929），以研究 NF-κB 通路对成纤维细胞增殖的影响。结果表明，与对照组相比，经等离子体处理少于 20s 的 L929 细胞增殖显著增加；随着 LTP 处理时间的延长，细胞内 ROS、O 和 NO 的产生增加；NF-κB 通路在适当的剂量范围内被 LTP 激活，并且 LTP 处理细胞中环素 D1 的表达与细胞增殖呈相同趋势增加。用 RNA 干扰阻断 p65 表达后，相同处理时间下，RNAi 处理的细胞增殖速度较慢，环素 D1 的表达也低于正常细胞。此外，用 N-乙酰-L-半胱氨酸（NAC）预处理可显著防止等离子体引起的细胞变化。总之，LTP 诱导的 L929 细胞增殖与 NF-κB 信号通路密切相关，该通路可能被适当水平的细胞内 ROS 激活。这些新发现可为 LTP 诱导细胞增殖和促进伤口愈合提供一些理论参考。
