Xu Gui-Min, Shi Xing-Min, Cai Jing-Fen, Chen Si-Le, Li Ping, Yao Cong-Wei, Chang Zheng-Shi, Zhang Guan-Jun
State Key Laboratory of Electrical Insulation and Power Equipment, School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.
Environment and Genes Related to Diseases Key Laboratory of Education Ministry, School of Public Health, Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.
Wound Repair Regen. 2015 Nov-Dec;23(6):878-84. doi: 10.1111/wrr.12364.
Cold plasma has become an attractive tool for promoting wound healing and treating skin diseases. This article presents an atmospheric pressure plasma jet (APPJ) generated in argon gas through dielectric barrier discharge, which was applied to superficial skin wounds in BALB/c mice. The mice (n = 50) were assigned randomly into five groups (named A, B, C, D, E) with 10 animals in each group. Natural wound healing was compared with stimulated wound healing treated daily with APPJ for different time spans (10, 20, 30, 40, and 50 seconds) on 14 consecutive days. APPJ emission spectra, morphological changes in animal wounds, and tissue histological parameters were analyzed. Statistical results revealed that wound size changed over the duration of the experimental period and there was a significant interaction between experimental day and group. Differences between group C and other groups at day 7 were statistically significant (p < 0.05). All groups had nearly achieved closure of the untreated control wounds at day 14. The wounds treated with APPJ for 10, 20, 30, and 40 seconds showed significantly enhanced daily improvement compared with the control and almost complete closure at day 12, 10, 7, and 13, respectively. The optimal results of epidermal cell regeneration, granulation tissue hyperplasia, and collagen deposition in histological aspect were observed at day 7. However, the wounds treated for 50 seconds were less well healed at day 14 than those of the control. It was concluded that appropriate doses of cold plasma could inactivate bacteria around the wound, activate fibroblast proliferation in wound tissue, and eventually promote wound healing. Whereas, over doses of plasma suppressed wound healing due to causing cell death by apoptosis or necrosis. Both positive and negative effects may be related to the existence of reactive oxygen and nitrogen species (ROS and RNS) in APPJ.
冷等离子体已成为促进伤口愈合和治疗皮肤病的一种有吸引力的工具。本文介绍了一种通过介质阻挡放电在氩气中产生的大气压等离子体射流（APPJ），并将其应用于BALB/c小鼠的浅表皮肤伤口。将50只小鼠随机分为五组（命名为A、B、C、D、E），每组10只。比较自然伤口愈合与连续14天每天用APPJ处理不同时间跨度（10、20、30、40和50秒）的刺激伤口愈合情况。分析了APPJ发射光谱、动物伤口的形态变化和组织学参数。统计结果显示，伤口大小在实验期间有所变化，且实验天数和组间存在显著交互作用。第7天C组与其他组之间的差异具有统计学意义（p<0.05）。在第14天，所有组未处理的对照伤口几乎都已闭合。与对照组相比，用APPJ处理10、20、30和40秒的伤口在每日改善方面显著增强，分别在第12、10、7和13天几乎完全闭合。在组织学方面，在第7天观察到表皮细胞再生、肉芽组织增生和胶原沉积的最佳结果。然而，在第14天，处理50秒的伤口愈合情况比对照组差。得出的结论是，适当剂量的冷等离子体可以使伤口周围的细菌失活，激活伤口组织中的成纤维细胞增殖，并最终促进伤口愈合。而过量的等离子体由于通过凋亡或坏死导致细胞死亡而抑制伤口愈合。正负两种效应可能都与APPJ中活性氧和氮物种（ROS和RNS）的存在有关。
