Key Laboratory of Bio-resources and Eco-environmaent of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, PR China.
Key Laboratory of Bio-resources and Eco-environmaent of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, PR China.
Peptides. 2017 Nov;97:1-7. doi: 10.1016/j.peptides.2017.09.009. Epub 2017 Sep 14.
The peptide hormone adropin, encoded by the energy homeostasis-associated (Enho) gene, plays a role in energy homeostasis and the control of vascular function. The aim of this study was to examine the role of adropin in growth hormone (GH) gene expression at the pituitary level in tilapia. As a first step, the antiserum for the tilapia adropin was produced, and its specificity was confirmed by antiserum preabsorption and immunohistochemical staining in the tilapia pituitary. Adropin could be detected immunocytochemically in the proximal pars distalis (PPD) of the tilapia pituitary. In primary cultures of tilapia pituitary cells, tilapia adropin was effective in increasing GH mRNA levels. However, removal of endogenous adropin by immunoneutralization using adropin antiserum inhibited GH gene expression. In parallel experiments, pituitary cells co-treated with ovine pituitary adenylate cyclase activating polypeptide 38 (oPACAP38) and adropin showed a similar increase level compared to those treated with oPACAP38 alone, whereas insulin-like growth factor 1 (IGF1) not only had an inhibitory effect on basal GH mRNA levels, but also could abolish adropin stimulation of GH gene expression. In pituitary cells pretreated with actinomycin D, the half-life of GH mRNA was enhanced by adropin. Taken together, these findings suggest that adropin may serve as a novel local stimulator for GH gene expression in tilapia pituitary.
肽激素阿多品由与能量平衡相关的（Enho）基因编码，在能量平衡和血管功能控制中发挥作用。本研究旨在研究阿多品在鱼类生长激素（GH）基因表达中的作用。作为第一步，产生了鱼类阿多品的抗血清，并通过抗血清预吸收和鱼类垂体的免疫组织化学染色证实了其特异性。阿多品可以在鱼类垂体的近侧部（PPD）免疫细胞化学检测到。在鱼类垂体细胞的原代培养中，鱼类阿多品可有效增加 GH mRNA 水平。然而，通过使用阿多品抗血清进行免疫中和去除内源性阿多品抑制了 GH 基因表达。在平行实验中，与单独用 oPACAP38 处理的细胞相比，用 oPACAP38 和阿多品共同处理的垂体细胞显示出相似的增加水平，而胰岛素样生长因子 1（IGF1）不仅对基础 GH mRNA 水平具有抑制作用，而且可以消除阿多品对 GH 基因表达的刺激。在用放线菌素 D 预处理的垂体细胞中，阿多品增强了 GH mRNA 的半衰期。总之，这些发现表明，阿多品可能作为鱼类垂体中 GH 基因表达的新型局部刺激物。
