菟丝子多糖逆转肾阳虚的纯化、鉴定及生物学作用
Purification, characterization and biological effect of reversing the kidney-yang deficiency of polysaccharides from semen cuscutae.
机构信息
School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing, 100102, China.
School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing, 100102, China; Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100029, China.
出版信息
Carbohydr Polym. 2017 Nov 1;175:249-256. doi: 10.1016/j.carbpol.2017.07.077. Epub 2017 Jul 27.
Semen cuscutae is a well-known Chinese medicine which has been used to nourish kidney. It is the first study to demonstrate that the polysaccharides from semen cuscutae showed significant activity of nourishing kidney-yang by increasing the levels of testosterone and estradiol, decreasing the level of blood urea nitrogen, improving immune function, possessing antioxidant effect. Three homogeneous polysaccharides were obtained by DEAE-cellulose and Sephacryl S-400 which were named as C-7WR1, C-7WR2 and C-7WR3 with average molecular weight of 7.59×10, 3.23×10 and 2.25×10 respectively. C-7WR1 was composed of fructose: mannose=0.02:1. C-7WR2 was composed of fructose: mannose: xylose: arabinose=0.01:1:0.14:0.33. C-7WR3 was composed of fructose: mannose: xylose: arabinose=0.01:1:0.10:0.47. They mainly contained mannose. Their fourier transform infrared features were similar. They all had no nucleic acid and protein.
菟丝子是一种众所周知的中药，具有补肾作用。本研究首次证明菟丝子多糖通过提高睾酮和雌二醇水平、降低血尿素氮水平、改善免疫功能、具有抗氧化作用，表现出显著的补肾阳活性。通过 DEAE-纤维素和 Sephacryl S-400 分离得到三种均一多糖，分别命名为 C-7WR1、C-7WR2 和 C-7WR3，其平均分子量分别为 7.59×10、3.23×10 和 2.25×10。C-7WR1 由果糖：甘露糖=0.02:1 组成。C-7WR2 由果糖：甘露糖：木糖：阿拉伯糖=0.01:1:0.14:0.33 组成。C-7WR3 由果糖：甘露糖：木糖：阿拉伯糖=0.01:1:0.10:0.47 组成。它们主要含有甘露糖。它们的傅里叶变换红外特征相似，均不含核酸和蛋白质。
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