哺乳动物视网膜中A II无长突细胞的多巴胺能神经支配。
Dopaminergic innervation of A II amacrine cells in mammalian retina.
作者信息
Voigt T, Wässle H
机构信息
Max-Planck-Institut für Hirnforschung, Frankfurt, West Germany.
出版信息
J Neurosci. 1987 Dec;7(12):4115-28. doi: 10.1523/JNEUROSCI.07-12-04115.1987.
Dopaminergic amacrine cells were stained in cat, rat, and rabbit retina using an antibody against tyrosine hydroxylase (TH). Following intraocular injection of DAPI (4,6,diamidino-2-phenylindole), subsequent retinal whole-mount preparations revealed that the dopaminergic fiber plexus formed rings around amacrine cell bodies. Intracellular injection of Lucifer yellow (LY) into A II amacrine cells confirmed that this rod-related, bistratified interneuron has its cell body within the dopaminergic rings. Using a photooxidation process, LY was transformed into an electron-dense reaction product, enabling ultrastructural examination of LY-injected A II amacrine cells. In retinae counterstained with an antibody against TH, it was possible to show synapses from TH-positive fibers onto these cells. The dopaminergic plexus was further investigated by injecting single dopaminergic cells with LY and thus revealing their branching pattern. The present results emphasize the role of dopamine in modulating the rod pathway in mammalian retina.
使用抗酪氨酸羟化酶（TH）抗体对猫、大鼠和兔视网膜中的多巴胺能无长突细胞进行染色。眼内注射4,6-二脒基-2-苯基吲哚（DAPI）后，随后的视网膜整装标本显示多巴胺能纤维丛在无长突细胞体周围形成环。向A II无长突细胞内注射荧光黄（LY）证实，这种与视杆相关的双分层中间神经元的细胞体位于多巴胺能环内。利用光氧化过程，LY被转化为电子致密反应产物，从而能够对注射了LY的A II无长突细胞进行超微结构检查。在用抗TH抗体进行复染的视网膜中，可以显示TH阳性纤维与这些细胞之间的突触。通过向单个多巴胺能细胞注射LY并揭示其分支模式，对多巴胺能丛进行了进一步研究。目前的结果强调了多巴胺在调节哺乳动物视网膜视杆通路中的作用。
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