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鲶鱼物种中一种类Tc1转座子的染色体定位

Chromosome mapping of a Tc1-like transposon in species of the catfish .

作者信息

da Silva Keteryne Rodrigues, Mariotto Sandra, Centofante Liano, Parise-Maltempi Patricia Pasquali

机构信息

Laboratório de Citogenética Animal - Universidade Estadual Paulista "Júlio de Mesquita Filho" Campus de Rio Claro - Av 24A, 1515 Jardim Bela Vista- 13600-000- Rio Claro/SP, Brasil.

Instituto Federal de Ciências e Tecnologia do Mato Grosso, campus de Cuiabá - Bela Vista, MT, Brasil.

出版信息

Comp Cytogenet. 2017 Jan 20;11(1):65-79. doi: 10.3897/CompCytogen.v11i1.10519. eCollection 2017.

Abstract

The Tc1 mariner element is widely distributed among organisms and have been already described in different species of fish. The genus (Kner, 1854) has 68 nominal species and is part of an interesting taxonomic and cytogenetic group, as well as presenting a variation of chromosome number, ranging from 2n=34 to 54 chromosomes, and the existence of simple and multiple sex chromosome system and the occurrence of chromosomal polymorphisms involving chromosomes that carry the nucleolus organizer region. In this study, a repetitive element by restriction enzyme, from sp.1 "Flecha" was isolated, which showed similarity with a transposable element Tc1-mariner. Its chromosomal location is distributed in heterochromatic regions and along the chromosomal arms of all specimens covered in this study, confirming the pattern dispersed of this element found in other studies carried out with other species. Thus, this result reinforces the hypothesis that the sequence AnDraI is really a dispersed element isolated. As this isolated sequence showed the same pattern in all species which have different sex chromosomes systems, including in all sex chromosomes, we could know that it is not involved in sex chromosome differentiation.

摘要

Tc1水手元件广泛分布于生物中,并且已经在不同鱼类物种中被描述。(克纳,1854年)属有68个命名物种,是一个有趣的分类学和细胞遗传学群体的一部分,同时呈现出染色体数目的变化,从2n = 34到54条染色体,存在简单和多重性染色体系统,以及涉及携带核仁组织区染色体的染色体多态性。在本研究中,从“Flecha”物种1中分离出一种经限制性内切酶作用的重复元件,其与转座元件Tc1-水手显示出相似性。它在染色体上的定位分布在异染色质区域以及本研究涵盖的所有标本的染色体臂上,证实了在对其他物种进行的其他研究中发现的该元件的分散模式。因此,这一结果强化了这样的假设,即序列AnDraI确实是一个分离出的分散元件。由于这个分离出的序列在所有具有不同性染色体系统的物种中,包括在所有性染色体中都显示出相同的模式,我们可以知道它不参与性染色体分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87b4/5599695/317377f2fd82/comparative_cytogenetics-11-065-g001.jpg

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