Liu Yuling, Liu Zhen, Peng Renhai, Wang Yuhong, Zhou Zhongli, Wang Xingxing, Zhang Zhenmei, Wang Kunbo, Liu Fang
Anyang Institute of Technology, Anyang, Henan, 455000, China.
State Key Laboratory of Cotton Biology / Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan, 455000, China.
Comp Cytogenet. 2017 Jun 19;11(2):405-420. doi: 10.3897/CompCytogen.v11i2.12824. eCollection 2017.
Cytogenetic maps of (Linnaeus, 1753) homoeologous chromosomes A01 and D01 were constructed by fluorescence hybridization (FISH), using eleven homoeologous-chromosomes-shared bacterial artificial chromosomes (BACs) clones and one chromosome-specific BAC clone respectively. We compared the cytogenetic maps with the genetic linkage and draft genome assembly maps based on a standardized map unit, relative map position (RMP), which allowed a global view of the relationship of genetic and physical distances along each chromosome, and assembly quality of the draft genome assembly map. By integration of cytogenetic maps with sequence maps of the two chromosomes (A01 and D01), we inferred the locations of two scaffolds and speculated that some homologous sequences belonging to homoeologous chromosomes were removed as repetitiveness during the sequence assembly. The result offers molecular tools for cotton genomics research and also provides valuable information for the improvement of the draft genome assembly.
通过荧光原位杂交（FISH）构建了（林奈，1753年）同源染色体A01和D01的细胞遗传图谱，分别使用了11个同源染色体共享的细菌人工染色体（BAC）克隆和1个染色体特异性BAC克隆。我们基于标准化图谱单位——相对图谱位置（RMP），将细胞遗传图谱与遗传连锁图谱和基因组草图组装图谱进行了比较，这使得我们能够全面了解每条染色体上遗传距离和物理距离之间的关系，以及基因组草图组装图谱的组装质量。通过将细胞遗传图谱与两条染色体（A01和D01）的序列图谱整合，我们推断了两个支架的位置，并推测在序列组装过程中，一些属于同源染色体的同源序列作为重复性序列被去除了。该结果为棉花基因组学研究提供了分子工具，也为改进基因组草图组装提供了有价值的信息。
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