Cui Xinglei, Liu Fang, Liu Yuling, Zhou Zhongli, Zhao Yanyan, Wang Chunying, Wang Xingxing, Cai Xiaoyan, Wang Yuhong, Meng Fei, Peng Renhai, Wang Kunbo
State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan 455000 China.
State Key Laboratory of Cotton Biology (China)/Institute of Cotton Research of Chinese Academy of Agricultural Science, Anyang, Henan 455000 China ; Anyang Institute of Technology, Anyang, Henan 455000 China.
Mol Cytogenet. 2015 Jan 22;8(1):2. doi: 10.1186/s13039-015-0106-y. eCollection 2015.
Cytogenetic map can provide not only information of the genome structure, but also can build a solid foundation for genetic research. With the developments of molecular and cytogenetic studies in cotton (Gossypium), the construction of cytogenetic map is becoming more and more imperative.
A cytogenetic map of chromosome 1 (A101) of Gossypium herbaceum (A1) which includes 10 bacterial artificial chromosome (BAC) clones was constructed by using fluorescent in situ hybridization (FISH). Meanwhile, comparison and analysis were made for the cytogenetic map of chromosome 1 (A101) of G. herbaceum with four genetic linkage maps of chromosome 1 (Ah01) of G. hirsutum ((AD)1) and one genetic linkage map of chromosome 1 of (A101) G. arboreum (A2). The 10 BAC clones were also used to be localized on G. raimondii (D5) chromosome 1 (D501), and 2 of them showed clear unique hybridized signals. Furthermore, these 2 BAC clones were also shown localized on chromosome 1 of both A sub-genome and D sub-genome of G. hirsutum.
The comparison of the cytogenetic map with genetic linkage maps showed that most of the identified marker-tagged BAC clones appearing same orders in different maps except three markers showing different positions, which might indicate chromosomal segmental rearrangements. The positions of the 2 BAC clones which were localized on Ah01 and Dh01 chromosomes were almost the same as that on A101 and D501 chromosomes. The corresponding anchored SSR markers of these 2 BAC clones were firstly found to be localized on chromosome D501 (Dh01) as they were not seen mapped like this in any genetic map reported.
细胞遗传图谱不仅能提供基因组结构信息，还可为遗传研究奠定坚实基础。随着棉花（棉属）分子和细胞遗传学研究的发展，构建细胞遗传图谱变得越来越迫切。
利用荧光原位杂交（FISH）构建了草棉（A1）1号染色体（A101）的细胞遗传图谱，该图谱包含10个细菌人工染色体（BAC）克隆。同时，将草棉1号染色体（A101）的细胞遗传图谱与陆地棉（(AD)1）1号染色体（Ah01）的四个遗传连锁图谱以及亚洲棉（A2）1号染色体（A101）的一个遗传连锁图谱进行了比较和分析。这10个BAC克隆也被用于定位在雷蒙德氏棉（D5）1号染色体（D501）上，其中2个显示出清晰独特的杂交信号。此外，这2个BAC克隆在陆地棉的A亚基因组和D亚基因组的1号染色体上也显示出定位。
细胞遗传图谱与遗传连锁图谱的比较表明，除了三个标记显示不同位置外，大多数已鉴定的标记标记BAC克隆在不同图谱中呈现相同顺序，这可能表明染色体片段重排。定位在Ah01和Dh01染色体上的2个BAC克隆的位置与在A101和D501染色体上的位置几乎相同。首次发现这2个BAC克隆的相应锚定SSR标记定位在D501（Dh01）染色体上，因为在任何已报道的遗传图谱中都没有这样的定位。
