纯血马中马ERBB受体反馈抑制剂1的基因表达与遗传变异特征分析
Characterization of gene expression and genetic variation of horse ERBB receptor feedback inhibitor 1 in Thoroughbreds.
作者信息
Choi Jae-Young, Jang Hyun-Jun, Park Jeong-Woong, Oh Jae-Don, Shin Donghyun, Kim Nam Young, Oh Jin Hyeog, Song Ki-Duk, Cho Byung-Wook
机构信息
Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea.
Department of Animal Biotechnology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju 54896, Korea.
出版信息
Asian-Australas J Anim Sci. 2018 Mar;31(3):309-315. doi: 10.5713/ajas.17.0370. Epub 2017 Sep 18.
OBJECTIVE
This study aimed to test the expression patterns of ERBB receptor feedback inhibitor 1 () before and after exercise and the association of non-synonymous single-nucleotide polymorphisms (nsSNPs) of horse with racing traits in Thoroughbreds.
METHODS
We performed bioinformatics and gene expression analyses for horse . Transcription factor (TF) binding sites in the 5'-regulatory region of this gene were identified through a tool for prediction of TF-binding site (PROMO). A general linear model was used to detect the association between the nsSNP (LOC42830758 A to G) and race performance.
RESULTS
Quantitative polymerase chain reaction analysis showed that expression level of after exercise was 1.6 times higher than that before exercise. Ten transcription factors were predicted from the regulatory region. A novel nsSNP (LOC42830758 A to G) was found in , which was associated with three racing traits including average prize money, average racing index, and 3-year-old starts percentile ranking.
CONCLUSION
Our analysis will be helpful as a basis for studying genes and SNPs that affect race performance in racehorses.
目的
本研究旨在测试运动前后ERBB受体反馈抑制剂1（ ）的表达模式，以及马的非同义单核苷酸多态性（nsSNPs）与纯种马比赛性状的关联。
方法
我们对马的 进行了生物信息学和基因表达分析。通过转录因子结合位点预测工具（PROMO）确定该基因5'调控区域中的转录因子（TF）结合位点。使用一般线性模型检测nsSNP（LOC42830758 A到G）与比赛成绩之间的关联。
结果
定量聚合酶链反应分析表明，运动后 的表达水平比运动前高1.6倍。从 调控区域预测出10个转录因子。在 中发现了一个新的nsSNP（LOC42830758 A到G），它与包括平均奖金、平均比赛指数和3岁马参赛百分排名在内的三个比赛性状相关。
结论
我们的分析将有助于作为研究影响赛马比赛成绩的基因和SNP的基础。
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