IEEE Trans Biomed Circuits Syst. 2017 Dec;11(6):1459-1469. doi: 10.1109/TBCAS.2017.2739724. Epub 2017 Sep 12.
This paper describes the design, fabrication, and testing of a microfluidic sensor for dielectric spectroscopy of human whole blood during coagulation. The sensor, termed ClotChip, employs a three-dimensional, parallel-plate, capacitive sensing structure with a floating electrode integrated into a microfluidic channel. Interfaced with an impedance analyzer, the ClotChip measures the complex relative dielectric permittivity, ϵ , of human whole blood in the frequency range of 40 Hz to 100 MHz. The temporal variation in the real part of the blood dielectric permittivity at 1 MHz features a time to reach a permittivity peak, , as well as a maximum change in permittivity after the peak, , as two distinct parameters of ClotChip readout. The ClotChip performance was benchmarked against rotational thromboelastometry (ROTEM) to evaluate the clinical utility of its readout parameters in capturing the clotting dynamics arising from coagulation factors and platelet activity. exhibited a very strong positive correlation ( r = 0.99, p < 0.0001) with the ROTEM clotting time parameter, whereas exhibited a strong positive correlation (r = 0.85, p < 0.001) with the ROTEM maximum clot firmness parameter. This paper demonstrates the ClotChip potential as a point-of-care platform to assess the complete hemostatic process using <10 μL of human whole blood.
本文描述了一种用于在凝血过程中对人体全血进行介电谱学检测的微流控传感器的设计、制造和测试。该传感器称为 ClotChip，采用了一种具有集成到微流道中的浮置电极的三维平行板电容式传感结构。ClotChip 与阻抗分析仪接口，可测量人体全血在 40 Hz 至 100 MHz 频率范围内的复介电常数 ϵ。在 1 MHz 时，血液介电常数实部的时间变化特征为达到介电常数峰值的时间 t p 和峰值后的介电常数最大变化量 Δε p ，这两个参数是 ClotChip 读数的两个不同参数。ClotChip 的性能与旋转血栓弹性测定法 (ROTEM) 进行了基准测试，以评估其读数参数在捕获凝血因子和血小板活性引起的凝血动力学方面的临床应用价值。t p 与 ROTEM 凝血时间参数具有非常强的正相关性（r = 0.99，p < 0.0001），而 Δε p 与 ROTEM 最大凝块硬度参数具有强正相关性（r = 0.85，p < 0.001）。本文证明了 ClotChip 作为一种使用<10 μL 人体全血即可评估完整止血过程的即时检测平台的潜力。
