Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, Brazil.
Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
Parasit Vectors. 2017 Sep 18;10(1):423. doi: 10.1186/s13071-017-2348-5.
Culex nigripalpus has a wide geographical distribution and is found in North and South America. Females are considered primary vectors for several arboviruses, including Saint Louis encephalitis virus, Venezuelan equine encephalitis virus and Eastern equine encephalitis virus, as well as a potential vector of West Nile virus. In view of the epidemiological importance of this mosquito and its high abundance, this study sought to investigate wing variation in Cx. nigripalpus populations from urban parks in the city of São Paulo, Brazil.
Female mosquitoes were collected in seven urban parks in the city of São Paulo between 2011 and 2013. Eighteen landmark coordinates from the right wing of each female mosquito were digitized, and the dissimilarities between populations were assessed by canonical variate analysis and cross-validated reclassification and by constructing a Neighbor-Joining (NJ) tree based on Mahalanobis distances. The centroid size was calculated to determine mean wing size in each population.
Canonical variate analysis based on fixed landmarks of the wing revealed a pattern of segregation between urban and sylvatic Cx. nigripalpus, a similar result to that revealed by the NJ tree topology, in which the population from Shangrilá Park segregated into a distinct branch separate from the other more urban populations.
Environmental heterogeneity may be affecting the wing shape variation of Cx. nigripalpus populations.
库蚊分布广泛，分布于北美洲和南美洲。雌性蚊子被认为是几种虫媒病毒的主要传播媒介，包括圣路易斯脑炎病毒、委内瑞拉马脑炎病毒和东部马脑炎病毒，以及西尼罗河病毒的潜在传播媒介。鉴于这种蚊子的流行病学重要性及其高丰度，本研究旨在调查巴西圣保罗市城市公园中库蚊种群的翅膀变异。
2011 年至 2013 年期间，在巴西圣保罗市的七个城市公园中收集了雌性蚊子。对每只雌性蚊子的右翼的 18 个标志坐标进行数字化，并通过典范变量分析和交叉验证重新分类以及构建基于马氏距离的邻接（NJ）树来评估种群之间的差异。计算质心大小以确定每个种群的平均翅膀大小。
基于翅膀固定标志的典范变量分析显示出城市和森林库蚊之间的分离模式，与 NJ 树拓扑结构揭示的结果相似，其中 Shangrilá 公园的种群分离成一个与其他更城市化种群分开的独特分支。
环境异质性可能影响库蚊种群的翅膀形状变异。
