外周血单核细胞在儿科葡萄膜炎中呈现激活表型。

Peripheral blood monocytes reveal an activated phenotype in pediatric uveitis.

机构信息

Department of Ophthalmology, Ophtha Lab, St. Franziskus-Hospital Muenster, Germany; Department of Pediatric Rheumatology and Immunology, University Hospital Muenster, Germany.

Department of Pediatric Rheumatology and Immunology, University Hospital Muenster, Germany.

出版信息

Clin Immunol. 2018 May;190:84-88. doi: 10.1016/j.clim.2017.09.014. Epub 2017 Sep 18.

DOI:10.1016/j.clim.2017.09.014

PMID:28923439

Abstract

OBJECTIVE

To characterize peripheral blood monocytes in uveitis associated with juvenile idiopathic arthritis (JIAU).

METHODS

Peripheral blood monocytes from children with JIA (either with (n = 18) or without uveitis (n = 11)), idiopathic anterior uveitis (IAU; n = 12) and healthy controls (n = 11) were analyzed by flow cytometry.

RESULTS

Percentage of CD14 + CD86+ monocytes and CD86 expression on single cell level were significantly higher in all patient groups than in controls, whereas no major differences existed between patient groups. Frequency of CD39+ (p < 0.05 all groups) and CD73+ monocytes (p = 0.03 JIAU vs controls) was elevated in patients. Disease activity did not influence monocyte phenotypes, but in methotrexate-treated JIAU patients numbers of CCR2+ monocytes were reduced and numbers of CD86+ and CD39+ cells increased.

CONCLUSION

Children with arthritis or uveitis display a distinct monocytic phenotype when compared to cells from healthy children. Phenotypic changes seem to be neither arthritis- nor uveitis-dependent, but may be modified by treatment.

摘要

目的

描述与幼年特发性关节炎（JIAU）相关的葡萄膜炎患者外周血单核细胞的特征。

方法

采用流式细胞术分析 JIA 患儿（伴发（n = 18）或不伴发葡萄膜炎（n = 11））、特发性前葡萄膜炎（IAU；n = 12）和健康对照者（n = 11）外周血单核细胞。

结果

与对照组相比，所有患者组的 CD14+CD86+单核细胞百分比和单细胞水平上的 CD86 表达均显著升高，而患者组之间无明显差异。CD39+（所有组均 p < 0.05）和 CD73+单核细胞（JIAU 与对照组相比 p = 0.03）的频率在患者中升高。疾病活动度未影响单核细胞表型，但在甲氨蝶呤治疗的 JIAU 患者中，CCR2+单核细胞数量减少，CD86+和 CD39+细胞数量增加。