Department of Ophthalmology, Ophtha-Lab at St. Franziskus Hospital, Münster, Germany.
University of Duisburg-Essen, Essen, Germany.
Ocul Immunol Inflamm. 2021 Aug 18;29(6):1080-1089. doi: 10.1080/09273948.2020.1715449. Epub 2020 Mar 11.
: To analyze circulating immune cells in patients with anterior uveitis (AU) associated to axial spondyloarthritis (SpA), or juvenile idiopathic arthritis (JIA).: Venous blood samples were collected from healthy controls (n = 16), and either SpA (n = 19) or JIA (n = 23) patients with associated anterior uveitis (AU) during active flare, or after ≥3 months of inactivity. Frequencies of CD56, MHC-I, and S100A9 monocytes, CCR7 dendritic cells, CD56 natural killer (NK) cells and CD3CD56 T-cells were analyzed via flow cytometry. Serum S100A8/A9 levels were determined via ELISA.: SpA patients showed a reduced frequency of CD56 NK cells during uveitis activity, a constitutively activated monocyte phenotype, and elevated S100A8/A9 serum levels. In contrast, JIAU patients showed elevated frequencies of CD56 monocytes and CCR7 DC.: Phenotype of peripheral immune cells differ between patients, probably contributing to different courses of acute onset AU in SpA and insidious onset AU in JIAU patients.: AU: anterior uveitis, AR: arthritis, JIA: juvenile idiopathic arthritis, SpA: axial spondyloarthritis.
分析与脊椎关节炎（SpA）或幼年特发性关节炎（JIA）相关的前葡萄膜炎（AU）患者的循环免疫细胞。
静脉血样取自健康对照者（n = 16），以及处于活动期前葡萄膜炎（AU）的 SpA 患者（n = 19）或 JIA 患者（n = 23），或 AU 缓解期（≥3 个月）。采用流式细胞术分析 CD56、MHC-I 和 S100A9 单核细胞、CCR7 树突状细胞、CD56 自然杀伤（NK）细胞和 CD3CD56 T 细胞的频率。采用 ELISA 法测定血清 S100A8/A9 水平。
SpA 患者在 AU 活动期时 NK 细胞频率降低，存在持续激活的单核细胞表型，且 S100A8/A9 血清水平升高。相比之下，JIAU 患者 CD56 单核细胞和 CCR7 DC 频率升高。
外周免疫细胞表型在患者间存在差异，可能导致 SpA 中急性发作 AU 和 JIAU 中隐匿性发作 AU 的不同病程。
: AU：前葡萄膜炎，AR：关节炎，JIA：幼年特发性关节炎，SpA：脊椎关节炎。
