Walscheid Karoline, Heiligenhaus Arnd, Holzinger Dirk, Roth Johannes, Heinz Carsten, Tappeiner Christoph, Kasper Maren, Foell Dirk
Department of Ophthalmology and Ophtha Lab St. Franziskus-Hospital Muenster, Muenster, Germany 2Department of Pediatric Rheumatology and Immunology, University Hospital Muenster, Muenster, Germany.
Department of Ophthalmology and Ophtha Lab St. Franziskus-Hospital Muenster, Muenster, Germany 3Department of Ophthalmology, University of Duisburg-Essen, Essen, Germany.
Invest Ophthalmol Vis Sci. 2015 Dec;56(13):7653-60. doi: 10.1167/iovs.15-17066.
Juvenile idiopathic arthritis-associated uveitis (JIAU) is the most common uveitis entity in childhood. As S100A8/A9 and S100A12 proteins are valuable biomarkers in childhood arthritis, we investigated the occurrence of these proteins in childhood uveitis.
Serum samples from patients with JIAU (n = 79) or idiopathic anterior uveitis (IAU, n = 24), as well as from nonuveitic controls (n = 24), were collected. Furthermore, aqueous humor samples (JIAU n = 17, nonuveitic controls n = 16, IAU n = 12) were obtained. Samples were analyzed for S100A8/A9 and S100A12 protein levels by ELISA. Intergroup comparisons were performed, involving patient data, clinical data, and S100 levels.
S100A8/A9 and S100A12 serum levels were elevated in IAU and JIAU patients as compared to nonuveitic controls (all P < 0.05). S100 serum levels in JIAU patients were higher in active arthritis (not significant; P = 0.289 for S100A8/A9 and P = 0.196 for S100A12) and active uveitis (P = 0.010 for S100A8/A9 and P = 0.026 for S100A12) than in controls. No significant differences in S100 levels were found in a subgroup analysis for sex, antinuclear antibody (ANA) status, disease duration, or presence of uveitis complications. In JIAU patients, S100 serum levels correlated with age and age at onset of uveitis. A longitudinal analysis in JIAU patients showed a correlation of serum S100A8/A9 and S100A12 levels with uveitis activity (both P = 0.03). S100A8/A9 levels in aqueous humor of patients with JIAU (P = 0.001) and IAU (P = 0.0002) were increased as compared to nonuveitic controls.
Increased S100A8/A9 and S100A12 levels are found in the serum and aqueous humor of patients with autoimmune uveitis. Serum levels reflect activity of joint and eye disease.
青少年特发性关节炎相关葡萄膜炎（JIAU）是儿童期最常见的葡萄膜炎类型。由于S100A8/A9和S100A12蛋白是儿童关节炎中有价值的生物标志物，我们研究了这些蛋白在儿童葡萄膜炎中的出现情况。
收集JIAU患者（n = 79）、特发性前葡萄膜炎（IAU，n = 24）患者以及非葡萄膜炎对照组（n = 24）的血清样本。此外，获取房水样本（JIAU组n = 17，非葡萄膜炎对照组n = 16，IAU组n = 12）。通过酶联免疫吸附测定法（ELISA）分析样本中的S100A8/A9和S100A12蛋白水平。进行组间比较，涉及患者数据、临床数据和S100水平。
与非葡萄膜炎对照组相比，IAU和JIAU患者的S100A8/A9和S100A12血清水平升高（所有P < 0.05）。JIAU患者在活动性关节炎（无显著性差异；S100A8/A9的P = 0.289，S100A12的P = 0.196）和活动性葡萄膜炎（S100A8/A9的P = 0.010，S100A12的P = 0.026）时的S100血清水平高于对照组。在按性别、抗核抗体（ANA）状态、病程或葡萄膜炎并发症存在情况进行的亚组分析中，未发现S100水平有显著差异。在JIAU患者中，S100血清水平与年龄以及葡萄膜炎发病年龄相关。对JIAU患者的纵向分析显示血清S100A8/A9和S100A12水平与葡萄膜炎活动度相关（两者P = 0.03）。与非葡萄膜炎对照组相比，JIAU患者（P = 0.001）和IAU患者（P = 0.0002）房水中的S100A8/A9水平升高。
自身免疫性葡萄膜炎患者的血清和房水中S100A8/A9和S100A12水平升高。血清水平反映关节和眼部疾病的活动度。
