Department of Ophthalmology and Ophtha-Laboratory at St. Franziskus Hospital, Münster, Germany.
University of Duisburg-Essen, Essen, Germany.
Front Immunol. 2018 Jul 30;9:1773. doi: 10.3389/fimmu.2018.01773. eCollection 2018.
HLA-B27 is the allele most frequently associated with human anterior uveitis. The majority of HLA-B27-positive [acute anterior uveitis (AAU)] patients develop clinically distinct symptoms with acute symptomatic onset of flare and a recurrent disease course characterized by a massive cellular ocular infiltrate during uveitis relapse. By contrast, uveitis in HLA-B27-negative [idiopathic anterior uveitis (IAU)] patients tends to develop a clinically less fulminant, more chronic, and typically asymptomatic disease course. To analyze systemic immune responses in the different uveitis entities, we analyzed peripheral blood cells by flow cytometry. In addition, as a pro-inflammatory biomarker serum, S100A8/A9 levels were quantified by ELISA from patients with AAU ( = 27) and IAU ( = 21), and in healthy controls ( = 30). Data were obtained either during active uveitis flare or after 3 months of inactivity. IAU patients showed a transiently increased frequency of CD56- and CD163-positive monocytes and of both granulocytic myeloid-derived suppressor cells and Th17 cells during active uveitis. By contrast, AAU patients showed an elevated frequency of monocytes, activated T cells, and elevated S100A8/A9 serum levels during clinically quiescent disease. The differentially regulated response of both innate and adaptive immune cells in the blood may be related to the clinically distinct characteristics of the two different uveitis entities.
HLA-B27 是与人类前葡萄膜炎最常相关的等位基因。大多数 HLA-B27 阳性 [急性前葡萄膜炎 (AAU)] 患者表现出明显不同的临床症状，表现为炎症发作时急性症状和复发性疾病过程，复发性疾病过程中葡萄膜炎复发时出现大量细胞性眼内浸润。相比之下，HLA-B27 阴性 [特发性前葡萄膜炎 (IAU)] 患者的葡萄膜炎往往表现为临床症状不那么剧烈、更慢性和通常无症状的疾病过程。为了分析不同葡萄膜炎实体中的全身免疫反应，我们通过流式细胞术分析了外周血细胞。此外，作为一种促炎生物标志物血清，我们通过 ELISA 从 AAU（ = 27）和 IAU（ = 21）患者以及健康对照者（ = 30）中定量了 S100A8/A9 水平。数据是在活动期葡萄膜炎发作期间或 3 个月无活动期后获得的。IAU 患者在活动期葡萄膜炎期间，CD56-和 CD163 阳性单核细胞以及粒细胞髓系来源的抑制细胞和 Th17 细胞的频率短暂增加。相比之下，AAU 患者在临床上静止疾病期间表现出单核细胞、活化 T 细胞和升高的 S100A8/A9 血清水平。血液中固有和适应性免疫细胞的差异调节反应可能与两种不同葡萄膜炎实体的临床特征明显不同有关。
